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Title:
LIVING CELL FREEZING METHOD AND LIVING CELL FREEZING SYSTEM
Document Type and Number:
WIPO Patent Application WO/2023/243709
Kind Code:
A1
Abstract:
Provided are a method and a living cell freezing system for freezing an unfrozen specimen comprising living cells in such a manner as to keep the cells alive. The method comprises: causing an unfrozen specimen to reach a frozen state by placing the unfrozen specimen, that includes a freezing solution containing a freezing protection agent and living cells immersed in the freezing solution, in an electrical field, generated by an electrode to which an alternating current with a frequency of 10-200 Hz is applied, so that said specimen is cooled while conduction electrical current flows. The electrical field is continuously generated without any interruption for a duration that the specimen goes from an unfrozen state to a frozen state. Also provided is a method for forming spheroids, the method comprising: freezing living cells having a spheroid-forming function by the foregoing freezing method; thawing the frozen living cells; and cultivating the thawed living cells to form spheroids.

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Inventors:
BAMBA KENZO (JP)
KOHARA ARIHIRO (JP)
OZAWA MIDORI (JP)
MORISHIMA YASUYUKI (JP)
HARA YUKO (JP)
SUMIKAWA HIROAKI (JP)
Application Number:
PCT/JP2023/022393
Publication Date:
December 21, 2023
Filing Date:
June 16, 2023
Export Citation:
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Assignee:
NAT INST BIOMEDICAL INNOVATION HEALTH & NUTRITION (JP)
SANTETSU ENG INC (JP)
LINKSU CO LTD (JP)
International Classes:
C12N1/04; C12N5/07; C12N5/0735; F25B21/00
Domestic Patent References:
WO2018185912A12018-10-11
Foreign References:
JP2007259709A2007-10-11
JP2011182700A2011-09-22
JP2006115837A2006-05-11
JP2017104061A2017-06-15
Other References:
IMAMURA SHINSUKE: "Cryopreservation of primate ES/iPS cells", BIOENGINEERING, vol. 90, 1 January 2012 (2012-01-01), pages 556 - 553, XP093118214
Attorney, Agent or Firm:
MIYOSHI Hidekazu et al. (JP)
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