Title:
LOW-MOLECULAR-WEIGHT CHONDROITIN SULFATE AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/081999
Kind Code:
A1
Abstract:
Disclosed are a low-molecular-weight chondroitin sulfate and a preparation method therefor. Macromolecular chondroitin sulfate is used as a raw material. A low-molecular-weight chondroitin sulfate having an average molecular weight of less than 1000 daltons can be obtained by means of a production process comprising chondroitin sulfate lyase degradation, deproteinization, filtration sterilization, drying, etc. With respect to the low-molecular-weight chondroitin sulfate, the molecular weight distribution range is narrow, the proportion of chondroitin sulfate disaccharide is 43％ to 60％, the proportion of chondroitin sulfate tetrasaccharide is 30％ to 45％, the sum of the contents of the chondroitin sulfate disaccharide and the chondroitin sulfate tetrasaccharide is greater than 87％, the total content of oligosaccharides in the low-molecular-weight chondroitin sulfate is not less than 97％, and the protein content is not more than 0.5％. Compared with common marketed macromolecular chondroitin sulfate, the product has, at a concentration of 50 μg/mL to 100 μg/mL, a more obvious repairing effect on chondrocytes damaged by 1 mM hydrogen peroxide, has a strong repairing ability and a repair rate between 14％ and 23％, and can be used to treat joint injuries, and same is thus an important raw material for medical products, healthcare products, cosmetics, food product, etc.
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Inventors:
WANG YIN (CN)
CUI HUAIYAN (CN)
LIANG XUGUANG (CN)
LI DEGENG (CN)
XU YONGGANG (CN)
TANG CHUANGEN (CN)
CHEN SONG (CN)
ZHANG HAONING (CN)
CUI HUAIYAN (CN)
LIANG XUGUANG (CN)
LI DEGENG (CN)
XU YONGGANG (CN)
TANG CHUANGEN (CN)
CHEN SONG (CN)
ZHANG HAONING (CN)
Application Number:
PCT/CN2019/115120
Publication Date:
May 06, 2021
Filing Date:
November 01, 2019
Export Citation:
Assignee:
NANJING HANXIN PHARMACEUTICAL TECH CO LTD (CN)
International Classes:
C12P19/14; A61K31/737; A61K35/60; C07H1/06; C08B37/08; C12P19/04
Foreign References:
| CN103623006A | 2014-03-12 | |||
| CN103623006A | 2014-03-12 | |||
| CN109913437A | 2019-06-21 | |||
| CN108070627A | 2018-05-25 | |||
| CN103602711A | 2014-02-26 | |||
| CN102676613A | 2012-09-19 | |||
| JP3278629B2 | 2002-04-30 | |||
| KR100706294B1 | 2007-04-11 |
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