Title:
LOW MOLECULAR WEIGHT CHONDROITIN SULFATE, COMPOSITION CONTAINING SAME, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/083384
Kind Code:
A1
Abstract:
Disclosed are a low molecular weight chondroitin sulfate and a preparation method therefor. The method comprises: using a macromolecular chondroitin sulfate as a raw material, and subjecting same to production processes of chondroitin sulfate lyase degradation, deproteinization, filtration, sterilization, drying, etc., so as to obtain a low molecular weight chondroitin sulfate with an average molecular weight less than 1000 daltons. The chondroitin sulfate has a narrow molecular weight distribution range, wherein chondroitin sulfate disaccharides account for 43-60%; chondroitin sulfate tetrasaccharides account for 30-45%; the sum of the content of chondroitin sulfate disaccharides and chondroitin sulfate tetrasaccharides is greater than 87%; the total content of oligosaccharides in the low molecular weight chondroitin sulfate is at least 97%; and the content of protein does not exceed 0.5%. Compared with common commercially available macromolecular chondroitin sulfate, the product has a more obvious repair effect on chondrocytes damaged by 1 mM hydrogen peroxide within the concentration of 50-100 μg/mL. The product has a strong repair ability with a repair rate between 14% and 23%, and can be used to treat joint injuries and is an important raw material for medical products, health care products, cosmetics, foods, etc.
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Inventors:
ZHANG HAONING (CN)
CHEN SONG (CN)
JIN BO (CN)
XU YONGGANG (CN)
TANG CHUANGEN (CN)
CHEN SONG (CN)
JIN BO (CN)
XU YONGGANG (CN)
TANG CHUANGEN (CN)
Application Number:
PCT/CN2020/126003
Publication Date:
May 06, 2021
Filing Date:
November 02, 2020
Export Citation:
Assignee:
NANJING XINLANG ENTERPRISE MAN CONSULTING CO LTD (CN)
International Classes:
C12P19/14; A61K31/737; A61K35/60; A61P19/02; C07H1/06; C08B37/08; C12P19/04
Foreign References:
| CN111741963A | 2020-10-02 | |||
| CN103623006A | 2014-03-12 | |||
| CN109913437A | 2019-06-21 | |||
| CN108070627A | 2018-05-25 | |||
| CN103602711A | 2014-02-26 | |||
| CN102676613A | 2012-09-19 | |||
| JP3278629B2 | 2002-04-30 | |||
| KR100706294B1 | 2007-04-11 | |||
| CN102676613A | 2012-09-19 | |||
| CN108070627A | 2018-05-25 | |||
| CN103602711A | 2014-02-26 | |||
| DE102019264385A | ||||
| CN109913437A | 2019-06-21 |
Other References:
DPPH 。, JING ZHANG, JING－LIANG ZHANG, CHANG－LIANG ZHU, YAN－HUI JIANG, ZHAO－PENG SHEN, XIAO－LU JIANG: "Chondroitin sulfate oligosaccharides prepared by chondroitinase and its antioxidant activities", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, GAI KAN BIANJIBU , BEIJING, CN, vol. 38, no. 13, 6 April 2017 (2017-04-06), CN, pages 48 - 52, XP055813148, ISSN: 1002-0306, DOI: 10． 13386 /j． issn1002 － 0306． 2017． 13． 009
"Preparation of Chondroitin Sulfate Oligosaccharides by Enzymatic Method and Its Antioxidant Activity", FOOD INDUSTRY SCIENCE AND TECHNOLOGY, vol. 13, 2017, pages 48 - 52
See also references of EP 4053290A4
"Preparation of Chondroitin Sulfate Oligosaccharides by Enzymatic Method and Its Antioxidant Activity", FOOD INDUSTRY SCIENCE AND TECHNOLOGY, vol. 13, 2017, pages 48 - 52
See also references of EP 4053290A4
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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