Title:
LUCIFERASE VARIANT
Document Type and Number:
WIPO Patent Application WO/2020/009215
Kind Code:
A1
Abstract:
In one embodiment, the present invention addresses the problem of providing a firefly luciferase having an improved thermal stability. In one embodiment, the present invention pertains to: a variant of firefly luciferase containing an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substituting the amino acid residue at the 393rd position, said luciferase variant having an improved thermal stability; a polynucleotide encoding the luciferase variant; and a method for producing the luciferase variant.
Inventors:
HAYASHI KANAKO (JP)
ICHIYANAGI ATSUSHI (JP)
ICHIYANAGI ATSUSHI (JP)
Application Number:
PCT/JP2019/026784
Publication Date:
January 09, 2020
Filing Date:
July 05, 2019
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N15/63; C12Q1/66
Domestic Patent References:
| WO2009066673A1 | 2009-05-28 |
Foreign References:
| JP2001516585A | 2001-10-02 | |||
| JP2010081908A | 2010-04-15 | |||
| JP2011120559A | 2011-06-23 |
Other References:
DEMENTIEVA, EI. ET AL.: "Physicochemical Properties of Recombinant Luciola mingrelica Luciferase and Its Mutant Forms", BIOCHEMISTRY, vol. 61, no. 1, 1996, pages 115 - 119, XP002195388
HATTORI N. ET AL.: "Mutant Luciferase Enzymes from Fireflies with Increased Resistance to Benzalkonium Chloride", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 66, no. 12, 2002, pages 2587 - 2593, XP008155238, DOI: 10.1271/bbb.66.2587
HATTORI N. ET AL.: "Mutant Luciferase Enzymes from Fireflies with Increased Resistance to Benzalkonium Chloride", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 66, no. 12, 2002, pages 2587 - 2593, XP008155238, DOI: 10.1271/bbb.66.2587
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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