Title:
LYTIC POLYSACCHARIDE MONOOXYGENASE FROM BACILLUS SUBTILIS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/016397
Kind Code:
A1
Abstract:
Provided is lytic polysaccharide monooxygenase BsLPMO10A from Bacillus subtilis. The amino acid sequence of the lytic polysaccharide monooxygenase is as shown in SEQ ID NO. 1. Further provided are a gene encoding the enzyme, an expression vector comprising the gene, a recombinant bacterium comprising the expression vector, and the use of the enzyme in promoting the degradation of hemicellulose substrates, promoting the rupture of β-1,4 glycosidic bonds of polysaccharide substrates, and synergistically promoting the decomposition of polysaccharides in combination with a glycoside hydrolase.
Inventors:
WANG QIAN (CN)
SUN XIAOBAO (CN)
WANG JIAKUN (CN)
LIU JIANXIN (CN)
SUN XIAOBAO (CN)
WANG JIAKUN (CN)
LIU JIANXIN (CN)
Application Number:
PCT/CN2022/111015
Publication Date:
January 25, 2024
Filing Date:
August 09, 2022
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12N9/02; C12N15/53; C12P19/14
Domestic Patent References:
| WO2018106656A1 | 2018-06-14 |
Foreign References:
| CN109957571A | 2019-07-02 |
Other References:
DATABASE Nucleotide 27 August 2015 (2015-08-27), ANONYMOUS : "Bacillus subtilis genome assembly Bacillus JRS10, contig contig000999, whole genome shotgun sequence ", XP093131658, retrieved from NCBI Database accession no. CYHR01000999.1
DATABASE Protein 28 July 2021 (2021-07-28), ANONYMOUS : "MULTISPECIES: lytic polysaccharide monooxygenase [Bacillus cereus group] ", XP093131659, retrieved from NCBI Database accession no. WP_078420274.1
XIAOBAO SUN, WAN JIAXIN, CAO JIAWEN, SI YUEXIU: "Progress in lytic polysaccharide monooxygenase", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 2, 25 February 2018 (2018-02-25), CN , pages 177 - 187, XP093131662, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170197
CORRÊA THAMY LÍVIA RIBEIRO, JÚNIOR ATÍLIO TOMAZINI, WOLF LÚCIA DANIELA, BUCKERIDGE MARCOS SILVEIRA, DOS SANTOS LEANDRO VIEIRA, MUR: "An actinobacteria lytic polysaccharide monooxygenase acts on both cellulose and xylan to boost biomass saccharification", BIOTECHNOLOGY FOR BIOFUELS, vol. 12, no. 1, 1 December 2019 (2019-12-01), pages 117, XP093131668, ISSN: 1754-6834, DOI: 10.1186/s13068-019-1449-0
DATABASE Protein 28 July 2021 (2021-07-28), ANONYMOUS : "MULTISPECIES: lytic polysaccharide monooxygenase [Bacillus cereus group] ", XP093131659, retrieved from NCBI Database accession no. WP_078420274.1
XIAOBAO SUN, WAN JIAXIN, CAO JIAWEN, SI YUEXIU: "Progress in lytic polysaccharide monooxygenase", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 2, 25 February 2018 (2018-02-25), CN , pages 177 - 187, XP093131662, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170197
CORRÊA THAMY LÍVIA RIBEIRO, JÚNIOR ATÍLIO TOMAZINI, WOLF LÚCIA DANIELA, BUCKERIDGE MARCOS SILVEIRA, DOS SANTOS LEANDRO VIEIRA, MUR: "An actinobacteria lytic polysaccharide monooxygenase acts on both cellulose and xylan to boost biomass saccharification", BIOTECHNOLOGY FOR BIOFUELS, vol. 12, no. 1, 1 December 2019 (2019-12-01), pages 117, XP093131668, ISSN: 1754-6834, DOI: 10.1186/s13068-019-1449-0
Attorney, Agent or Firm:
CHENGDU FANGYU UNION PATENT AGENCY (GENERAL PARTNERSHIP) (CN)
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