Title:
METHOD OF AMYNOACYLATING tRNA
Document Type and Number:
WIPO Patent Application WO/2004/022744
Kind Code:
A1
Abstract:
It is intended to provide a method of chemically synthesizing an aminoacyl tRNA which is completely different from the existing methods, namely, a highly efficient and practically usable method of synthesizing an aminoacyl tRNA whereby any unnatural amino acid can by conveniently aminoacylated without a need for genetic engineering techniques and detected without resort to a radioactive isotope. The above-described aminoacylation method comprises enclosing a tRNA and an amino acid in the vicinity of the interface of a micelle and bringing them close to each other to thereby react the same, or providing between them a peptide nucleic acid specifically binding to the tRNA as an antisense molecule and bringing them close to each other to thereby react the same.
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Inventors:
SISIDO MASAHIKO (JP)
NINOMIYA KEIKO (JP)
NINOMIYA KEIKO (JP)
Application Number:
PCT/JP2003/011391
Publication Date:
March 18, 2004
Filing Date:
September 05, 2003
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
SISIDO MASAHIKO (JP)
NINOMIYA KEIKO (JP)
SISIDO MASAHIKO (JP)
NINOMIYA KEIKO (JP)
International Classes:
C07H21/02; (IPC1-7): C12N15/09; C07H21/02
Other References:
KEIKO NINOMIYA ET AL.: "Antisense PNA yudotai ni yoru tRNA amino acyl-ka no kento", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1F310), vol. 79, no. 2, 15 March 2001 (2001-03-15), pages 873, XP002975115
WERNER M. ET AL.: "Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P", RNA, vol. 4, no. 7, July 1998 (1998-07-01), pages 847 - 855, XP002975116
SISIDO M. ET AL.: "Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into protein through four-base codon/anticodon pairs", APPL. MICROBIOL. BIOTECHNOL., vol. 57, no. 3, October 2001 (2001-10-01), pages 274 - 281, XP002975117
CHIKA KURITA ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu (3) cation-sei micelle-chu deno pdCpA oyobi tRNA no amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-39), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975118
TOSHIKAZU MINOHATA ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu(1) PNA ni ketsugo shita kasseika aminosan ni yoru tRNA no amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-37), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975119
MAJA SUZUKI ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu(2), PNA ni ketsugo shita kasseika aminosan-chugata DNA-tRNA: sangenso deno amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-38), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975120
See also references of EP 1548109A4
WERNER M. ET AL.: "Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P", RNA, vol. 4, no. 7, July 1998 (1998-07-01), pages 847 - 855, XP002975116
SISIDO M. ET AL.: "Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into protein through four-base codon/anticodon pairs", APPL. MICROBIOL. BIOTECHNOL., vol. 57, no. 3, October 2001 (2001-10-01), pages 274 - 281, XP002975117
CHIKA KURITA ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu (3) cation-sei micelle-chu deno pdCpA oyobi tRNA no amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-39), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975118
TOSHIKAZU MINOHATA ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu(1) PNA ni ketsugo shita kasseika aminosan ni yoru tRNA no amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-37), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975119
MAJA SUZUKI ET AL.: "tRNA tokuiteki na kagakuteki amino acyl-kaho no kaihatsu(2), PNA ni ketsugo shita kasseika aminosan-chugata DNA-tRNA: sangenso deno amino acyl-ka", THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU (1G2-38), vol. 83, no. 2, 3 March 2003 (2003-03-03), pages 944, XP002975120
See also references of EP 1548109A4
Attorney, Agent or Firm:
Saeki, Norio (Aminosan Kaikan Building 15-8, Nihonbashi 3-chom, Chuo-ku Tokyo, JP)
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