Title:
METHOD FOR DETECTING MITOCHONDRIAL tRNA MODIFICATION
Document Type and Number:
WIPO Patent Application WO/2018/124235
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for detecting a modified nucleoside of mitochondrial transition RNA (mt-tRNA). The present invention provides a method for detecting a modified nucleoside in mt-tRNA, the method using tandem mass analysis, and including detecting a modified nucleoside (for example, 5-taurinomethyl-2-thiouridine (τ m5s2U)), 5-taurinomethyluridine (τ m5U), or 2-methylthio-N6-isopentenyl adenosine (ms2i6A)) in a specimen of a bodily fluid such as urine or in a specimen in a culture supernatant.
Inventors:
TOMIZAWA KAZUHITO (JP)
WEI FANYAN (JP)
WEI FANYAN (JP)
Application Number:
PCT/JP2017/047099
Publication Date:
July 05, 2018
Filing Date:
December 27, 2017
Export Citation:
Assignee:
UNIV KUMAMOTO NAT UNIV CORP (JP)
International Classes:
G01N27/62; C12Q1/68; G01N33/50
Foreign References:
| JP2014513528A | 2014-06-05 |
Other References:
TARKOWSKI, PETR: "Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry", ANALYTICA CHIMICA ACTA, vol. 680, no. 1-2, 8 November 2010 (2010-11-08), pages 86 - 91, XP027428289, DOI: doi:10.1016/j.aca.2010.09.020
SUZUKI, TAKEO: "Structural determination and biosynthetic mechanism for novel modified uridine that exists in mammal mitochondrion tRNA", 23 March 2018 (2018-03-23), Retrieved from the Internet
CAO, XIAOYU: "Enhanced Detection of Post-Transcriptional Modifications Using a Mass-Exclusion List Strategy for RNA Modification Mapping by LC-MS/MS", ANALYTICAL CHEMISTRY, vol. 87, no. 16, 15 July 2015 (2015-07-15), pages 8433 - 8440, XP055607072
SUZUKI, TAKEO: "Structural determination and biosynthetic mechanism for novel modified uridine that exists in mammal mitochondrion tRNA", 23 March 2018 (2018-03-23), Retrieved from the Internet
CAO, XIAOYU: "Enhanced Detection of Post-Transcriptional Modifications Using a Mass-Exclusion List Strategy for RNA Modification Mapping by LC-MS/MS", ANALYTICAL CHEMISTRY, vol. 87, no. 16, 15 July 2015 (2015-07-15), pages 8433 - 8440, XP055607072
Attorney, Agent or Firm:
OSAWA Kenichi (JP)
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