Title:
METHOD AND KIT FOR QUANTIFYING small, dense LDL CHOLESTEROL
Document Type and Number:
WIPO Patent Application WO/2021/049518
Kind Code:
A1
Abstract:
Provided are: a method for suppressing the natural coloration of a reagent during storage in a method for quantifying cholesterol in small, dense LDL in two steps using an autoanalyzer without pre-treating the sample; a quantification kit to be used in this method; and a preparation method. A kit for quantifying small, dense LDL cholesterol in a test sample that includes: (1) a first reagent composition having cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity, the first reagent composition being for leading cholesterol in lipoproteins other than small, dense LDL outside the reaction system in the presence of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity; and (2) a second reagent composition for quantifying the cholesterol in the small, dense LDL, the kit being used in a method for quantifying cholesterol in small, dense LDL in two steps, wherein the kit is characterized in that a coupler, an iron complex, and peroxidase activity are not jointly present in the same reagent composition in either the first reagent composition or the second reagent composition.
Inventors:
ITOH YASUKI (JP)
SATOH NORIYUKI (JP)
SATOH NORIYUKI (JP)
Application Number:
PCT/JP2020/034078
Publication Date:
March 18, 2021
Filing Date:
September 09, 2020
Export Citation:
Assignee:
DENKA COMPANY LTD (JP)
International Classes:
C12Q1/26; C12Q1/28; C12Q1/30; C12Q1/34; C12Q1/44; G01N33/92
Domestic Patent References:
| WO2009048143A1 | 2009-04-16 | |||
| WO2004053500A1 | 2004-06-24 |
Foreign References:
| JP2005278626A | 2005-10-13 | |||
| JP2005292110A | 2005-10-20 | |||
| JP2003227798A | 2003-08-15 | |||
| JP2003028882A | 2003-01-29 | |||
| JPS5111389B2 | 1976-04-10 | |||
| JP5450080B2 | 2014-03-26 | |||
| JPS5325093B2 | 1978-07-25 | |||
| JP2019164403A | 2019-09-26 |
Other References:
JAMA, vol. 260, 1988, pages 1917 - 21
"HANDBOOK OF LIPOPROTEIN TESTING", 2002, AACC PRESS, article "The Fats of Life", pages: 609 - 623
LVDD 15 YEAR ANNIVERSARY ISSUE, vol. AVI, no. 3, pages 15 - 16
ATHEROSCLEROSIS, vol. 106, 1994, pages 241 - 253
ATHEROSCLEROSIS, vol. 83, 1990, pages 59
See also references of EP 4029948A4
"HANDBOOK OF LIPOPROTEIN TESTING", 2002, AACC PRESS, article "The Fats of Life", pages: 609 - 623
LVDD 15 YEAR ANNIVERSARY ISSUE, vol. AVI, no. 3, pages 15 - 16
ATHEROSCLEROSIS, vol. 106, 1994, pages 241 - 253
ATHEROSCLEROSIS, vol. 83, 1990, pages 59
See also references of EP 4029948A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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