Title:
METHOD FOR PRODUCING PROTEIN HAVING Fc REGION BY ALKALI WASHING
Document Type and Number:
WIPO Patent Application WO/2016/031932
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for purifying a protein having an Fc region having reduced problems with properties when purifying a protein having an Fc region having problems with properties such as free thiol content, self-association, and cohesiveness. The free thiol content is decreased and self-association and cohesiveness are also decreased by conducting a step for washing by an alkali wash solution in a state in which a protein having an Fc region is bonded on a carrier for affinity chromatography and refining the protein having an Fc region.
Inventors:
YAMANOUCHI MASAYA (JP)
IMURA YUICHI (JP)
TAGAWA TOSHIAKI (JP)
NONOYAMA SATOSHI (JP)
FUJINO YASUHIRO (JP)
IMURA YUICHI (JP)
TAGAWA TOSHIAKI (JP)
NONOYAMA SATOSHI (JP)
FUJINO YASUHIRO (JP)
Application Number:
PCT/JP2015/074286
Publication Date:
March 03, 2016
Filing Date:
August 27, 2015
Export Citation:
Assignee:
MITSUBISHI TANABE PHARMA CORP (JP)
International Classes:
C07K1/22; C07K1/113; C07K16/00; C12P21/08; C12N15/09
Domestic Patent References:
| WO2015099175A1 | 2015-07-02 |
Foreign References:
| JP2013504621A | 2013-02-07 | |||
| JPH01135798A | 1989-05-29 | |||
| JPH02290900A | 1990-11-30 | |||
| JP2014515389A | 2014-06-30 |
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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