Title:
METHOD FOR mRNA STABILIZATION
Document Type and Number:
WIPO Patent Application WO/2019/093423
Kind Code:
A1
Abstract:
A complex of mRNA encoding a target gene with RNA oligomers, wherein the RNA oligomers are at least two kinds of RNA oligomers comprising RNA sequences of (a) or (b), the at least two kinds of RNA oligomers are hybridizable respectively with sequences, said sequences being different from each other, in the mRNA sequence, and the complex is formed when a first sequence of the RNA oligomers hybridizes with different mRNA from the second sequence of the RNA oligomers: (a) an RNA sequence which comprises a first sequence consisting of 12-40 bases and being complementary to the mRNA sequence, a linker sequence consisting of 0-100 bases and a sequence which is the same as the first sequence, in this order; and (b) an RNA sequence which comprises a first sequence, which has a 90% or more identity with a sequence consisting of 12-40 bases and being complementary to the mRNA sequence and is hybridizable with mRNA, a linker sequence consisting of 0-100 bases and a second sequence which is the same as the first sequence, in this order. Thus, a novel method for mRNA stabilization is provided.
Inventors:
UCHIDA SATOSHI (JP)
YOSHINAGA NAOTO (JP)
CHO EOL (JP)
SHOJI KYOKO (JP)
YOSHINAGA NAOTO (JP)
CHO EOL (JP)
SHOJI KYOKO (JP)
Application Number:
PCT/JP2018/041493
Publication Date:
May 16, 2019
Filing Date:
November 08, 2018
Export Citation:
Assignee:
UNIV TOKYO (JP)
KAWASAKI INST OF INDUSTRIAL PROMOTION (JP)
KAWASAKI INST OF INDUSTRIAL PROMOTION (JP)
International Classes:
C12N15/11; A61K31/7088; A61K31/7115; A61K48/00; A61P31/04; A61P31/10; A61P31/12; A61P33/00; A61P35/00; A61P37/04
Domestic Patent References:
| WO2007001986A2 | 2007-01-04 | |||
| WO2018124181A1 | 2018-07-05 | |||
| WO2015121924A1 | 2015-08-20 |
Foreign References:
| JP2017505623A | 2017-02-23 | |||
| JP2014527819A | 2014-10-23 | |||
| JP2015529469A | 2015-10-08 | |||
| US20130023433A1 | 2013-01-24 | |||
| US201762583691P | 2017-11-09 |
Other References:
CHO, EOL ET AL.: "Messenger aiming to improve enzyme heat resistance, Design of RNA nano-assemblies", PREPRINTS OF THE 39TH CONFERENCE OF JAPANESE SOCIETY FOR BIOMATERIALS; NOVEMBER 20-21, 2017, 21 November 2017 (2017-11-21), pages 148, XP009520294
See also references of EP 3708664A4
MEIS, J. E.CHEN, F., EPICENTRE FORUM, vol. 9, no. 1, 2002, pages 10
UCHIDA, S. ET AL., BIOMATERIALS, vol. 82, 2016, pages 221 - 228
BLOOD, vol. 108, no. 13, 2006, pages 4009 - 17
ALTZSHUL S.F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403
OSAWA, S. ET AL., BIOMACROMOLECULES, vol. 17, 2016, pages 354 - 361
PITTELA ET AL., BIOMATERIALS, vol. 32, 2011, pages 3106 - 3114
MIYATA, K. ET AL., BIOMATERIALS, vol. 31, 2010, pages 4764 - 4770
See also references of EP 3708664A4
MEIS, J. E.CHEN, F., EPICENTRE FORUM, vol. 9, no. 1, 2002, pages 10
UCHIDA, S. ET AL., BIOMATERIALS, vol. 82, 2016, pages 221 - 228
BLOOD, vol. 108, no. 13, 2006, pages 4009 - 17
ALTZSHUL S.F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403
OSAWA, S. ET AL., BIOMACROMOLECULES, vol. 17, 2016, pages 354 - 361
PITTELA ET AL., BIOMATERIALS, vol. 32, 2011, pages 3106 - 3114
MIYATA, K. ET AL., BIOMATERIALS, vol. 31, 2010, pages 4764 - 4770
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
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