Title:
MONOCLONAL DES-LYS<58>-beta2-MICROGLOBULIN ANTIBODIES FOR MEASUREMENT AND MANAGEMENT OF HEMODIALYSIS COMPLICATIONS
Document Type and Number:
WIPO Patent Application WO2005063335
Kind Code:
A3
Abstract:
beta2-microglobulin (beta2m) is susceptible to specific cleavage after and subsequent removal of its Lysine-58 amino acid residue in various chronic disease conditions. This has been shown by incubating patient sera with normal beta2m and following structural alterations by electrophoretic techniques. At present, only very elaborate analytical methods can be used to analyze the state of beta2m in a complex sample matrix such as a serum sample because no specific reagent, e.g. an antibody, is available. The present invention provides antibodies that are specific for the cleaved variant of beta2m, des Lys<58>-beta2-microglobulin, in free solution in biological fluids. These antibodies may be used in methods for detecting des-Lys<58->beta2-microglobulin and in particular in in vitro diagnostic procedures. In addition to such methods, the invention provides antibodies for use as medicaments and for the preparation of medicaments as well as a method for generating the antibodies, devices for reducing the content of des-Lys<58>-beta2-microglobulin in a given material such as a hemodialysis fluid and, finally, in vitro diagnostic kits.
Inventors:
HEEGAARD NIELS HENRIK HELWEG (DK)
CORLIN DORTHE BIANCA (DK)
CORLIN DORTHE BIANCA (DK)
Application Number:
PCT/DK2004/000895
Publication Date:
September 29, 2005
Filing Date:
December 22, 2004
Export Citation:
Assignee:
STATENS SERUMINSTITUT (DK)
HEEGAARD NIELS HENRIK HELWEG (DK)
CORLIN DORTHE BIANCA (DK)
HEEGAARD NIELS HENRIK HELWEG (DK)
CORLIN DORTHE BIANCA (DK)
International Classes:
C07K16/28; G01N33/53; G01N33/68; (IPC1-7): A61P13/12; A61K39/395; A61P29/00; A61P35/00; A61P37/00; C07K16/28; G01N33/50
Foreign References:
| EP0229723A2 | 1987-07-22 | |||
| EP0236509A1 | 1987-09-16 |
Other References:
TACHIBANA K ET AL: "A TWO-SITE SANDWICH RADIOIMMUNOASSAY OF BETA2-MICROGLOBULIN WITH MONOCLONAL ANTIBODIES", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 75, no. 1, 1984, pages 43 - 51, XP001199532, ISSN: 0022-1759
NIELS HH HEEGAARD ET AL.: "Cleaved beta-2-microglobulin partially attains a conformation that has amyloidogenic features", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 13, 2002, USA, pages 11184 - 11189, XP002293806
JW SEN ET AL.: "On-line immunoafinity-liquid chromatography-mass spectrometry for identification of amyloid disease markers in biological fluids.", ANALYTICAL CHEMISTRY, vol. 75, no. 5, 2003, pages 1196 - 1202, XP002294180
ANONYMOUS: "B2-microglobulin antibodies", INTERNET ARTICLE, XP002293807, Retrieved from the Internet [retrieved on 20040825]
NIELS HH HEEGAARD ET AL.: "Cleaved beta-2-microglobulin partially attains a conformation that has amyloidogenic features", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 13, 2002, USA, pages 11184 - 11189, XP002293806
JW SEN ET AL.: "On-line immunoafinity-liquid chromatography-mass spectrometry for identification of amyloid disease markers in biological fluids.", ANALYTICAL CHEMISTRY, vol. 75, no. 5, 2003, pages 1196 - 1202, XP002294180
ANONYMOUS: "B2-microglobulin antibodies", INTERNET ARTICLE, XP002293807, Retrieved from the Internet
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