Title:
MAGNETIC BEAD, AND PREPARATION METHOD THEREFOR AND USE THEREOF IN NUCLEIC ACID EXTRACTION
Document Type and Number:
WIPO Patent Application WO/2023/125592
Kind Code:
A1
Abstract:
A magnetic bead, and a preparation method therefor and the use thereof in nucleic acid extraction. The preparation method for a magnetic bead comprises: preparing a magnetic core by means of a water-soluble salt of Fe2+ and Fe3+; and subjecting the magnetic core and a silanide to a modification reaction to obtain the magnetic bead, wherein the molar ratio of the addition amount of the used silanide to the total amount of Fe2+ and Fe3+ is greater than or equal to 0.5 : 1, and the particle size range of the magnetic bead is 0.1-50 μm.
Inventors:
SUN LIWEI (CN)
LIU JUNCHENG (CN)
JIN PENG (CN)
LIU SIWEN (CN)
ZHOU LIANG (CN)
ZHANG SUYA (CN)
LIU JUNCHENG (CN)
JIN PENG (CN)
LIU SIWEN (CN)
ZHOU LIANG (CN)
ZHANG SUYA (CN)
Application Number:
PCT/CN2022/142572
Publication Date:
July 06, 2023
Filing Date:
December 28, 2022
Export Citation:
Assignee:
NANJING GENSCRIPT BIOTECH CO LTD (CN)
International Classes:
C12N15/10; B01J20/10; H01F1/42
Domestic Patent References:
| WO2021198502A1 | 2021-10-07 | |||
| WO2021198289A1 | 2021-10-07 |
Foreign References:
| CN114395553A | 2022-04-26 | |||
| CN105647910A | 2016-06-08 | |||
| CN105420230A | 2016-03-23 | |||
| CN105561914A | 2016-05-11 | |||
| CN112563016A | 2021-03-26 | |||
| CN101979541A | 2011-02-23 | |||
| CN105821034A | 2016-08-03 | |||
| CN113004546A | 2021-06-22 |
Other References:
OBERACKER PHIL, STEPPER PETER, BOND DONNA, HIPP KATHARINA, HORE TIMOTHY, JURKOWSKI TOMASZ: "Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation", BIO-PROTOCOL, BIO-PROTOCOL.ORG, SUNNYVALE, CA, USA, vol. 9, no. 20, 1 January 2019 (2019-01-01), Sunnyvale, CA, USA , XP093074779, ISSN: 2331-8325, DOI: 10.21769/BioProtoc.3394
AJINKYA NENE, YU XUEFENG, KAITHAL POONAM, LUO HONGRONG, SOMANI PRAKASH, RAMAKRISHNA SEERAM: "Magnetic Iron Oxide Nanoparticle (IONP) Synthesis to Applications: Present and Future", MATERIALS, vol. 13, no. 20, pages 4644, XP093074782, DOI: 10.3390/ma13204644
BERENSMEIER SONJA: "Magnetic particles for the separation and purification of nucleic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 73, no. 3, 25 October 2006 (2006-10-25), Berlin/Heidelberg, pages 495 - 504, XP037066395, ISSN: 0175-7598, DOI: 10.1007/s00253-006-0675-0
FAN, Q.Q. ET AL.: "A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction", CHEMICAL PHYSICS LETTERS, vol. 715, 2 November 2018 (2018-11-02), XP085576693, DOI: 10.1016/j.cplett.2018.11.001
LI HAI-YANG, FEI WANG, HONG-TAO LEI, ZHANG XUAN, CHEN, KE: "Optimization of Genomic DNA Extraction with Silica Hydroxyl Magnetic Beads", BIOTECHNOLOGY BULLETIN = SHENGWU JISHU TONGBAO, BEIJING, vol. 33, no. 6, 1 January 2017 (2017-01-01), Beijing, pages 223 - 229, XP093054739, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2016-1045
AJINKYA NENE, YU XUEFENG, KAITHAL POONAM, LUO HONGRONG, SOMANI PRAKASH, RAMAKRISHNA SEERAM: "Magnetic Iron Oxide Nanoparticle (IONP) Synthesis to Applications: Present and Future", MATERIALS, vol. 13, no. 20, pages 4644, XP093074782, DOI: 10.3390/ma13204644
BERENSMEIER SONJA: "Magnetic particles for the separation and purification of nucleic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 73, no. 3, 25 October 2006 (2006-10-25), Berlin/Heidelberg, pages 495 - 504, XP037066395, ISSN: 0175-7598, DOI: 10.1007/s00253-006-0675-0
FAN, Q.Q. ET AL.: "A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction", CHEMICAL PHYSICS LETTERS, vol. 715, 2 November 2018 (2018-11-02), XP085576693, DOI: 10.1016/j.cplett.2018.11.001
LI HAI-YANG, FEI WANG, HONG-TAO LEI, ZHANG XUAN, CHEN, KE: "Optimization of Genomic DNA Extraction with Silica Hydroxyl Magnetic Beads", BIOTECHNOLOGY BULLETIN = SHENGWU JISHU TONGBAO, BEIJING, vol. 33, no. 6, 1 January 2017 (2017-01-01), Beijing, pages 223 - 229, XP093054739, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2016-1045
Attorney, Agent or Firm:
ZHONG LUN LAW FIRM (CN)
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