Title:
MAMMALIAN ORTHOGONAL ENZYMES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/244612
Kind Code:
A1
Abstract:
Provided is a method for analyzing a target using a labeled target-binding substance, wherein at least one enzyme selected from the group consisting of α-arabinofuranosidase, α-rhamnosidase, β-xylosidase, α-sulfoquinovosidase, α-galacturonidase, β-galacturonidase, and α-glucuronidase, or both β-glucosidase and at least one enzyme selected from the group consisting of the aforementioned enzymes, are used as a labeling material.
Inventors:
KATAYAMA YOSHIKI (JP)
MORI TAKESHI (JP)
MORI TAKESHI (JP)
Application Number:
PCT/JP2022/019229
Publication Date:
November 24, 2022
Filing Date:
April 28, 2022
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C07K16/00; C12N9/14; C12Q1/34; G01N33/536
Foreign References:
| US20060094031A1 | 2006-05-04 | |||
| JPH07270421A | 1995-10-20 | |||
| JP2002511770A | 2002-04-16 | |||
| US20150125888A1 | 2015-05-07 |
Other References:
BERLIN W, SAUER B: "In situ color detection of alpha-L-arabinofuranosidase, a "No-background" reporter gene, with 5-bromo-3-indolyl-alpha-L-arabino furanoside", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 243, no. 1, 1 January 1996 (1996-01-01), Amsterdam, NL , pages 171 - 175, XP002271582, ISSN: 0003-2697, DOI: 10.1006/abio.1996.0497
KANEKO RYOSUKE, ODA TSUYOSHI, YOSHIDA RYOSUKE, TATEISHI CHUYA, TANITO KENTA, NII TERUKI, KISHIMURA AKIHIRO, KAMIYA NORIHO, MORI TA: "α-L-Arabinofuranosidase as an Orthogonal Enzyme for Human Cells", CHEMISTRY LETTERS, CHEMICAL SOCIETY OF JAPAN,NIPPON KAGAKUKAI, JP, vol. 50, no. 8, 17 July 2021 (2021-07-17), JP , pages 1493 - 1495, XP093006274, ISSN: 0366-7022, DOI: 10.1246/cl.210231
KANEKO RYOSUKE, ODA TSUYOSHI, YOSHIDA RYOSUKE, TATEISHI CHUYA, TANITO KENTA, NII TERUKI, KISHIMURA AKIHIRO, KAMIYA NORIHO, MORI TA: "α-L-Arabinofuranosidase as an Orthogonal Enzyme for Human Cells", CHEMISTRY LETTERS, CHEMICAL SOCIETY OF JAPAN,NIPPON KAGAKUKAI, JP, vol. 50, no. 8, 17 July 2021 (2021-07-17), JP , pages 1493 - 1495, XP093006274, ISSN: 0366-7022, DOI: 10.1246/cl.210231
Attorney, Agent or Firm:
SIKS & CO. (JP)
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