MARKER FREE TRANSGENIC PLANTS: ENGINEERING THE CHLOROPLAST GENOME WITHOUT THE USE OF ANTIBIOTIC SELECTION

Title:

MARKER FREE TRANSGENIC PLANTS: ENGINEERING THE CHLOROPLAST GENOME WITHOUT THE USE OF ANTIBIOTIC SELECTION

Document Type and Number:

WIPO Patent Application WO2001064023

Kind Code:

B1

Abstract:

The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.

Inventors:

DANIELL HENRY (US)

Application Number:

PCT/US2001/006275

Publication Date:

December 13, 2001

Filing Date:

February 28, 2001

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Assignee:

UNIV AUBURN (US)
UNIV CENTRAL FLORIDA (US)
DANIELL HENRY (US)

International Classes:

C12N15/82; (IPC1-7): A01H5/00; C12N5/04; C12N15/29; C12N15/31; C12N15/52; C12N15/82

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