Title:
MATERIAL FOR HYDROXYLATING HYDROCARBON-BASED SUBSTRATE, AND USE OF MATERIAL
Document Type and Number:
WIPO Patent Application WO/2017/061624
Kind Code:
A1
Abstract:
This disclosure provides: a first site that is a terminal structure capable of binding a decoy molecule, for hydroxylating a hydrocarbon-based substrate by a cytochrome P450 monooxygenase, to a substrate-binding site of the cytochrome P450 monooxygenase; a second site that is a non-fluorinated alkyl chain; and a linker that includes a peptide bond and that links the terminal structure and the alkyl chain.
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Inventors:
SHOJI OSAMI (JP)
WATANABE YOSHIHITO (JP)
WATANABE YOSHIHITO (JP)
Application Number:
PCT/JP2016/080026
Publication Date:
April 13, 2017
Filing Date:
October 07, 2016
Export Citation:
Assignee:
NAT UNIV CORP NAGOYA UNIV (JP)
International Classes:
C07C233/47; C12P7/02; C12P7/06; C12Q1/26; C12N9/02
Foreign References:
| JP2012024009A | 2012-02-09 |
Other References:
DONOVAN C. HAINES ET AL.: "A single active-site mutation of P450BM-3 dramatically enhances substrate binding and rate of product formation.", BIOCHEMISTRY, vol. 50, 2011, pages 8333 - 8341, XP055372277
DONOVAN C. HAINES ET AL.: "Crystal structure of inhibitor-bound P450BM-3 reveals open conformation of substrate access channel.", BIOCHEMISTRY, vol. 47, 2008, pages 3662 - 3670, XP055372279
AMITA HEDGE ET AL.: "Interactions of substrates at the surface of P450s can greatly enhance substrate potency.", BIOCHEMISTRY, vol. 46, 2007, pages 14010 - 14017, XP055372281
ZHIQI CONG ET AL.: "Activation of wild-type cytochlome P450BM3 by the next generation of decoy molecules: enhanced hydroxylation of gaseous alkanes and crystallographic evidence.", ACS CATALYSIS, vol. 5, November 2014 (2014-11-01), pages 150 - 156, XP055372282
DONOVAN C. HAINES ET AL.: "Crystal structure of inhibitor-bound P450BM-3 reveals open conformation of substrate access channel.", BIOCHEMISTRY, vol. 47, 2008, pages 3662 - 3670, XP055372279
AMITA HEDGE ET AL.: "Interactions of substrates at the surface of P450s can greatly enhance substrate potency.", BIOCHEMISTRY, vol. 46, 2007, pages 14010 - 14017, XP055372281
ZHIQI CONG ET AL.: "Activation of wild-type cytochlome P450BM3 by the next generation of decoy molecules: enhanced hydroxylation of gaseous alkanes and crystallographic evidence.", ACS CATALYSIS, vol. 5, November 2014 (2014-11-01), pages 150 - 156, XP055372282
Attorney, Agent or Firm:
KAI-U PATENT LAW FIRM (JP)
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