Title:
MEDIUM COMPOSITION FOR CULTURING SELF-ACTIVATED LYMPHOCYTES AND METHOD FOR CULTURING SELF-ACTIVATED LYMPHOCYTES USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/030057
Kind Code:
A2
Abstract:
The present invention relates to a medium composition for culturing self-activated lymphocytes applicable to the treatment of a malignant tumor, and a method for culturing self-activated lymphocytes using the same, and more specifically, to a medium composition for culturing self-activated lymphocytes which effectively amplifies and activates natural killer (NK) cells, T cells and NKT cells by adding anti-CD3 and anti-CD16 antibodies in addition to interleukin-2 (IL-2), interleukin-12 (IL-12) and interleukin-18 (IL-18) to a medium, and can culture immunocytes which are remarkably effective for the treatment of various types of malignant tumors by increasing the relative ratio of NK cells among lymphocytes by leaps and bounds, and a method for culturing self-activated lymphocytes using the same. According to the present invention, the method for culturing self-activated lymphocytes comprises the following steps: extracting lymphocytes from peripheral human blood; first, culturing the extracted lymphocytes in a culture solution containing interleukin-2 (IL-2), L-glutamine and self-induced plasma in the presence of interleukin-12 (IL-12), interleukin-18 (IL-18), and anti-CD3 and anti-CD16 antibodies; and mixing the culture mixture solution cultured in the first culturing step with a culture solution containing IL-2, L-glutamine and self-induced plasma and second, culturing the obtained mixture solution in the presence of IL-12, an anti-CD16 antibody and IL-18.
Inventors:
HONG SEON MIN (KR)
BAEK YOUNG SEOK (KR)
BAEK YOUNG SEOK (KR)
Application Number:
PCT/KR2011/004013
Publication Date:
March 08, 2012
Filing Date:
June 01, 2011
Export Citation:
Assignee:
NKBIO CO LTD (KR)
HONG SEON MIN (KR)
BAEK YOUNG SEOK (KR)
HONG SEON MIN (KR)
BAEK YOUNG SEOK (KR)
International Classes:
C12N5/078; C07K14/54; C07K16/28; C12N5/02
Other References:
PINTARIC, M. ET AL.: 'Synergistic effects of IL-2, IL-12 and IL-18 on cytolytic activity perform expression and IFN-g production of porcine natural killer cells' VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY vol. 121, 2008, pages 68 - 82
RODRIGUEZ-GALAN, M.C. ET AL.: 'Synergistic Effect of IL-2, IL-12, and IL-18 on Thymocyte Apoptosis and Thl/Th2 Cytokine Expression' THE JOURNAL OF IMMUNOLOGY vol. 174, 2005, pages 2796 - 2804
SATWANI, P. ET AL.: 'Enhanced expansion, activation and cytolytic activity of umbilical cord blood (UCB) cytolytic T lymphocytes cultured in interleukin (IL)-18, IL-2, IL12 and anti-CD3: A functional analysis' PROC AMER AS COC CANCER RES vol. 45, 2004, page 4712
AGAUGUE, S. ET AL.: 'Human natural killer cells exposed to IL-2, IL-12, IL-18, or IL-4 differently modulate priming of naive T cells by monocyte-derived dendritic cells' BLOOD vol. 112, no. 5, 01 September 2008, pages 1776 - 1783
HONG, S-M. ET AL.: 'Human activated lymphocyte treated with anti-CD3, CD16, CD56 monoclonal antibody and IL-2' IMMUNE NETWORK vol. 5, no. 1, 2005, pages 11 - 15
RODRIGUEZ-GALAN, M.C. ET AL.: 'Synergistic Effect of IL-2, IL-12, and IL-18 on Thymocyte Apoptosis and Thl/Th2 Cytokine Expression' THE JOURNAL OF IMMUNOLOGY vol. 174, 2005, pages 2796 - 2804
SATWANI, P. ET AL.: 'Enhanced expansion, activation and cytolytic activity of umbilical cord blood (UCB) cytolytic T lymphocytes cultured in interleukin (IL)-18, IL-2, IL12 and anti-CD3: A functional analysis' PROC AMER AS COC CANCER RES vol. 45, 2004, page 4712
AGAUGUE, S. ET AL.: 'Human natural killer cells exposed to IL-2, IL-12, IL-18, or IL-4 differently modulate priming of naive T cells by monocyte-derived dendritic cells' BLOOD vol. 112, no. 5, 01 September 2008, pages 1776 - 1783
HONG, S-M. ET AL.: 'Human activated lymphocyte treated with anti-CD3, CD16, CD56 monoclonal antibody and IL-2' IMMUNE NETWORK vol. 5, no. 1, 2005, pages 11 - 15
Attorney, Agent or Firm:
SHIN, Uncheol (KR)
신운철 (KR)
신운철 (KR)
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