Title:
METABOLIC ENZYME-INDUCED MICRO-NANO PLASTIC PARTICLE BIODEGRADATION METHOD AND PRODUCT ANALYSIS METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/160841
Kind Code:
A1
Abstract:
Provided are a metabolic enzyme-induced micro-nano plastic particle biodegradation method and a product analysis method therefor, comprising the following steps: step (1), taking an adequate amount of plastic products and cutting into small pieces; step (2), placing in a ball mill tank and vacuum milling in a ball mill; step (3), weighing a milled plastic sample, dispersing using water, and preparing a dispersion of a certain concentration; step (4), mixing the micro-nano plastic dispersion with a metabolic enzyme solution, placing the mixture in a vortex mixer, and mixing evenly; step (5), placing the mixture in a water bath incubator for incubation. Provided is a method for micro-nano plastics to be induced by a biological metabolic enzyme—glutathione S-transferase in mild conditions into being biodegraded and metabolized.
Inventors:
LIU QIAN (CN)
HUANG XIU (CN)
JIANG GUIBIN (CN)
HUANG XIU (CN)
JIANG GUIBIN (CN)
Application Number:
PCT/CN2021/128825
Publication Date:
August 04, 2022
Filing Date:
November 04, 2021
Export Citation:
Assignee:
RES CT FOR ECO ENVIRONMENTAL SCIENCES CAS (CN)
International Classes:
G01N1/38; C12Q1/48; G01N27/623
Foreign References:
| CN112903805A | 2021-06-04 | |||
| CN103170089A | 2013-06-26 | |||
| US20140275436A1 | 2014-09-18 | |||
| US20190345472A1 | 2019-11-14 | |||
| CN109900774A | 2019-06-18 |
Other References:
SHEHU DAYYABU, ALIAS ZAZALI: "Dechlorination of polychlorobiphenyl degradation metabolites by a recombinant glutathione S-transferase from Acidovorax sp. KKS102", FEBS OPEN BIO, ELSEVIER, US, vol. 9, no. 3, 18 November 2017 (2017-11-18), US , pages 408 - 419, XP055954671, ISSN: 2211-5463, DOI: 10.1002/2211-5463.12405
SAMAK NADIA A.; JIA YUNPU; SHARSHAR MOUSTAFA M.; MU TINGZHEN; YANG MAOHUA; PEH SUMIT; XING JIANMIN: "Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling", ENVIRONMENT INTERNATIONAL., PERGAMON PRESS., US, vol. 145, 25 September 2020 (2020-09-25), US , XP086301339, ISSN: 0160-4120, DOI: 10.1016/j.envint.2020.106144
LUO WEI: "Progress on Co-metabolism of Biorefractory Pollutants", CHINESE JOURNAL OF SOIL SCIENCE, vol. 43, no. 6, 1 December 2012 (2012-12-01), pages 1 - 7, XP055954674, DOI: 10.19336/j.cnki.trtb.2012.06.041
SAMAK NADIA A.; JIA YUNPU; SHARSHAR MOUSTAFA M.; MU TINGZHEN; YANG MAOHUA; PEH SUMIT; XING JIANMIN: "Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling", ENVIRONMENT INTERNATIONAL., PERGAMON PRESS., US, vol. 145, 25 September 2020 (2020-09-25), US , XP086301339, ISSN: 0160-4120, DOI: 10.1016/j.envint.2020.106144
LUO WEI: "Progress on Co-metabolism of Biorefractory Pollutants", CHINESE JOURNAL OF SOIL SCIENCE, vol. 43, no. 6, 1 December 2012 (2012-12-01), pages 1 - 7, XP055954674, DOI: 10.19336/j.cnki.trtb.2012.06.041
Attorney, Agent or Firm:
BEIJING DADI ZHIGU INTELLECTUAL PROPERTY AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
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