Title:
A METAL-REDUCING ENZYMATIC TAG FOR OPTICAL AND ELECTRON MICROSCOPY
Document Type and Number:
WIPO Patent Application WO/2020/036831
Kind Code:
A3
Abstract:
Enzymes that reduce specific metal or metalloid ions to insoluble form are important to science. Peptides isolated from yeast- and phage-display libraries can affect the size and morphology of inorganic materials during their synthesis. Herein, an Se binding peptide was fused to an enzyme capable of reducing selenite (SeO32-) to a Se0 nanoparticle (SeNP). The fusion of the Se binding peptide to the metalloid reductase provided size control of the resulting SeNP. The SeNP product also remains associated to the enzyme fusion. The Se binding peptide fusion to the enzyme increases the enzyme's SeO32- reductase activity. Modification of enzyme activity was absent, and the size control of particles was diminished when the Se binding peptide was added exogenously to the reaction mixture. Binding of the peptide is attributed to His based ligation that results in a conformational change to the peptide.
Inventors:
ACKERSON CHRISTOPHER J (US)
BUTZ ZACHARY (US)
NEMETH RICHARD (US)
RISKOWSKI RYAN (US)
BORGOGNONI KANDA (US)
BUTZ ZACHARY (US)
NEMETH RICHARD (US)
RISKOWSKI RYAN (US)
BORGOGNONI KANDA (US)
Application Number:
PCT/US2019/046027
Publication Date:
May 28, 2020
Filing Date:
August 09, 2019
Export Citation:
Assignee:
UNIV COLORADO STATE RES FOUND (US)
ACKERSON CHRISTOPHER J (US)
BUTZ ZACHARY (US)
NEMETH RICHARD (US)
RISKOWSKI RYAN (US)
BORGOGNONI KANDA (US)
ACKERSON CHRISTOPHER J (US)
BUTZ ZACHARY (US)
NEMETH RICHARD (US)
RISKOWSKI RYAN (US)
BORGOGNONI KANDA (US)
International Classes:
C12Q1/26; C12Q1/00; G01N27/327; G01N33/15
Domestic Patent References:
| WO2006084276A2 | 2006-08-10 | |||
| WO2020036831A2 | 2020-02-20 |
Foreign References:
| US20030059955A1 | 2003-03-27 |
Other References:
NI ET AL.: "Progress toward clonable inorganic nanoparticles", NANOSCALE, vol. 7, no. 41, 9 September 2015 (2015-09-09), pages 17320 - 17327, XP055711028, DOI: 10.1039/C5NR04097C
Attorney, Agent or Firm:
HORVATH, Raymond F. (US)
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