METHOD FOR ACHIEVING TAG-TO-TAA CONVERSION ON GENOME WITH HIGH THROUGHPUT

WIPO Patent Application WO/2023/050169

A1

Provided is a method for achieving TAG-to-TAA conversion on a genome with high throughput. According to the method, by co-transfecting a gRNA array pool or a transcription product thereof, a plasmid containing a mCherry-inactivated eGFP reporter molecule and a sgRNA plasmid for editing and activating eGFP into a stable cell of an inducible base editor, or by transfecting an expression vector 43-all-in-one or a transcription product thereof into the stable cell of the inducible base editor, TAG-to-TAA conversion with high throughput in a single cell is realized, and almost all TAG-to-TAA conversions in a genome can be implemented after multiple cycles of operation.

JP3064065	INDUSTRIAL APPLICABILITY: Method for producing NlaIII-restricted endonuclease and methylase
WO/2024/073775	COMPOSITIONS AND METHODS FOR ENHANCING ADOPTIVE T CELL THERAPEUTICS
WO/2023/283622	CRISPR-BASED PROGRAMMABLE RNA EDITING

CHEN YUTING (CN)

PCT/CN2021/121750

April 06, 2023

September 29, 2021

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SHENZHEN INST ADV TECH (CN)
SHENZHEN INST OF ADV TECH CAS (CN)

C12N9/22; C12N15/10

CN112805379A	2021-05-14
CN110747186A	2020-02-04
CN110959040A	2020-04-03
US20210108188A1	2021-04-15

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ZHANG, TINGTING ET AL.: "Programmable base editing efficiency study of CRISPR/Cas9-guided DNA base editors in pig genome", HUBEI AGRICULTURAL SCIENCES, vol. 59, no. 18, 30 September 2020 (2020-09-30), XP009545016

BEIJING CHENGHUI LAW FIRM (CN)

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