Title:
METHOD FOR ACTIVATING AND PROLIFERATING CD8 T CELLS EXPOSED TO ANTIGEN, CD8 T CELLS, PREPARED THEREBY, HAVING ENHANCED ANTICANCER ACTIVITY, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/220412
Kind Code:
A1
Abstract:
The present invention relates to a cell activating method, activated cells thereby, and a use thereof. More specifically, the present invention provides: a method for functional enhancement, recovery, and proliferation of CD8 T cells of an exhausted state in an in-vitro condition, the method comprising a step of inducing overexpression of Klf4 protein in CD8 T cells isolated from individuals, a cell population including the CD8 T cells, or CAR-CD8 T cells genetically transformed from the CD8 T cells; CD8 T cells having anticancer activity, in which Klf4 protein is overexpressed by the method; and a use thereof.
Inventors:
SEONG RHO HYUN (KR)
NAH JIN WOO (KR)
NAH JIN WOO (KR)
Application Number:
PCT/KR2022/003482
Publication Date:
October 20, 2022
Filing Date:
March 11, 2022
Export Citation:
Assignee:
MEDGENE THERAPEUTICS INC (US)
International Classes:
C12N5/0783; A61K35/17; A61P31/00; A61P35/00; C07K14/47; C07K14/705
Domestic Patent References:
| WO2020200303A1 | 2020-10-08 |
Foreign References:
| KR20200113284A | 2020-10-06 | |||
| KR20200027508A | 2020-03-12 | |||
| US8106092B2 | 2012-01-31 | |||
| US20190218515A1 | 2019-07-18 |
Other References:
WU YANPING, LIN LIANJUN, WANG XIANG, LI YONG, LIU ZHONGHUI, YE WEI, HUANG WEIMING, LIN GANG, LIU HAIBO, ZHANG JIXIN, LI TING, ZHAO: "Overexpression of Krüppel-Like Factor 4 Suppresses Migration and Invasion of Non-Small Cell Lung Cancer Through c-Jun-NH2-Terminal Kinase/Epithelial-Mesenchymal Transition Signaling Pathway", FRONTIERS IN PHARMACOLOGY, vol. 10, 8 January 2020 (2020-01-08), XP055977229, DOI: 10.3389/fphar.2019.01512
JIE AN, SUSANNE GOLECH, JETTANONG KLAEWSONGKRAM, GAIL HUSTON, WILLIAM WOOD, YONGQING ZHANG, ROBERT WERSTO, KEVIN BECKER, SUSAN SWA: "Role of Kruppel-like factor 4(Klf4) in regulation of T cell number and TH-17 differentiation(139.12)", THE JOURNAL OF IMMUNOLOGY, WILLIAMS & WILKINS CO., US, vol. 184, no. 1 supplement, 1 April 2010 (2010-04-01), US , pages 139.12, XP009540295, ISSN: 0022-1767
HARTMANN ET AL., EMBO MOL. MED, vol. 9, no. 9, 2017, pages 1183 - 1197
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GYURIS ET AL., CELL, vol. 75, 1995, pages 791 - 803
GLOCKSHUBER ET AL., BIOCHEM, vol. 29, no. 6, 1990, pages 1362 - 1367
NYGREN, P. A., FEES J, vol. 275, no. 11, 2008, pages 2668 - 2676
EBERSBACH ET AL., J. MOL. BIOL., vol. 372, no. 1, 2007, pages 172 - 185
JOHNSON ET AL., ANAL. CHEM, vol. 84, no. 15, 2012, pages 6553 - 6560
KREHENBRINK ET AL., J. MOL. BIOL, vol. 383, no. 5, 2008, pages 1058 - 1068
DESMET ET AL., NAT. COMMUN, vol. 23, no. 12, 2005, pages 1556 - 1561
STUMPP ET AL., DRUG DISCOV. TODAY, vol. 13, no. 15-16, 2008, pages 695 - 701
GRABULOVSKI ET AL., J. BIOL. CHEM., vol. 282, no. 5, 2007, pages 3196 - 3204
NIXONWOOD, CURR. OPIN. DRUG DISCOV. DEV, vol. 9, no. 2, 2006, pages 261 - 268
KOIDEKOIDE, METHODS MOL. BIOL, vol. 352, 2007, pages 95 - 109
SUDERMAN ET AL., PROTEIN EXP. PURIF, vol. 134, 2017, pages 114 - 124
BOEHM ET AL., ANN. REV. IMMUNOL, vol. 30, 2012, pages 203 - 220
LEE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 109, 2012, pages 3299 - 3304
"Remington's Pharmaceutical Sciences", 1975, MACK PUBLISHING COMPANY
CERCEK ET AL., INVEST. NEW. DRUG, vol. 33, no. 5, 2015, pages 1086 - 1092
PARRY ET AL., J. IMMUNOL, vol. 171, 2003, pages 166 - 174
DULL ET AL., J. VIROL, vol. 72, 1998, pages 8463 - 8471
CARPENITO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 106, 2009, pages 3360 - 3365
PARRY ET AL., J. IMMUNOL., vol. 171, 2003, pages 166 - 174
BRENTJENS ET AL., SCI. TRANSL. MED, vol. 5, 2013, pages 177ra38
BRENTJENS, R. L ET AL., BLOOD, vol. 118, 2011, pages 4817 - 4823
JIE AN, SUSANNE GOLECH, JETTANONG KLAEWSONGKRAM, GAIL HUSTON, WILLIAM WOOD, YONGQING ZHANG, ROBERT WERSTO, KEVIN BECKER, SUSAN SWA: "Role of Kruppel-like factor 4(Klf4) in regulation of T cell number and TH-17 differentiation(139.12)", THE JOURNAL OF IMMUNOLOGY, WILLIAMS & WILKINS CO., US, vol. 184, no. 1 supplement, 1 April 2010 (2010-04-01), US , pages 139.12, XP009540295, ISSN: 0022-1767
HARTMANN ET AL., EMBO MOL. MED, vol. 9, no. 9, 2017, pages 1183 - 1197
CHARRON ET AL., J. BIOL. CHEM., vol. 270, 1995, pages 25739 - 25745
PAGANO ET AL., SCIENCE, vol. 255, 1992, pages 1144 - 1147
GYURIS ET AL., CELL, vol. 75, 1995, pages 791 - 803
GLOCKSHUBER ET AL., BIOCHEM, vol. 29, no. 6, 1990, pages 1362 - 1367
NYGREN, P. A., FEES J, vol. 275, no. 11, 2008, pages 2668 - 2676
EBERSBACH ET AL., J. MOL. BIOL., vol. 372, no. 1, 2007, pages 172 - 185
JOHNSON ET AL., ANAL. CHEM, vol. 84, no. 15, 2012, pages 6553 - 6560
KREHENBRINK ET AL., J. MOL. BIOL, vol. 383, no. 5, 2008, pages 1058 - 1068
DESMET ET AL., NAT. COMMUN, vol. 23, no. 12, 2005, pages 1556 - 1561
STUMPP ET AL., DRUG DISCOV. TODAY, vol. 13, no. 15-16, 2008, pages 695 - 701
GRABULOVSKI ET AL., J. BIOL. CHEM., vol. 282, no. 5, 2007, pages 3196 - 3204
NIXONWOOD, CURR. OPIN. DRUG DISCOV. DEV, vol. 9, no. 2, 2006, pages 261 - 268
KOIDEKOIDE, METHODS MOL. BIOL, vol. 352, 2007, pages 95 - 109
SUDERMAN ET AL., PROTEIN EXP. PURIF, vol. 134, 2017, pages 114 - 124
BOEHM ET AL., ANN. REV. IMMUNOL, vol. 30, 2012, pages 203 - 220
LEE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 109, 2012, pages 3299 - 3304
"Remington's Pharmaceutical Sciences", 1975, MACK PUBLISHING COMPANY
CERCEK ET AL., INVEST. NEW. DRUG, vol. 33, no. 5, 2015, pages 1086 - 1092
PARRY ET AL., J. IMMUNOL, vol. 171, 2003, pages 166 - 174
DULL ET AL., J. VIROL, vol. 72, 1998, pages 8463 - 8471
CARPENITO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 106, 2009, pages 3360 - 3365
PARRY ET AL., J. IMMUNOL., vol. 171, 2003, pages 166 - 174
BRENTJENS ET AL., SCI. TRANSL. MED, vol. 5, 2013, pages 177ra38
BRENTJENS, R. L ET AL., BLOOD, vol. 118, 2011, pages 4817 - 4823
Attorney, Agent or Firm:
HAN, Yunho (KR)
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