Title:
METHOD FOR AMPLIFYING NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2021/141108
Kind Code:
A1
Abstract:
Provided is a method for amplifying a nucleic acid from RNA with high sensitivity and a high yield. This method for amplifying a nucleic acid comprises: synthesizing cDNA from sample RNA through a reverse transcription reaction; and amplifying the cDNA by multiplex PCR, wherein the reverse transcription and the multiplex PCR are performed in the presence of a single-stranded nucleic acid-binding protein, and the single-stranded nucleic acid-binding protein is T4GP32 or a variant thereof.
Inventors:
UEHARA YUYA (JP)
INOUE TAKAYOSHI (JP)
INOUE TAKAYOSHI (JP)
Application Number:
PCT/JP2021/000457
Publication Date:
July 15, 2021
Filing Date:
January 08, 2021
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C07K14/01; C12N15/33; C12Q1/686
Domestic Patent References:
| WO2018008319A1 | 2018-01-11 | |||
| WO2018008319A1 | 2018-01-11 | |||
| WO2016052619A1 | 2016-04-07 |
Foreign References:
| JP2009535053A | 2009-10-01 | |||
| JP2019043040W | 2019-11-01 |
Other References:
PICHÉ CAROLINE, SCHERNTHANER JOHANN P: "Optimization of in Vitro Transcription and Full-Length cDNA Synthesis Using the T4 Bacteriophage Gene 32 Protein", JOURNAL OF BIOMOLECULAR TECHNIQUES, vol. 16, no. 3, 2005, pages 239 - 247, XP055841349
CHANDLER, D. P. ET AL.: "Reverse Transcriptase (RT) Inhibition of PCR at Low Concentrations of Template and Its Implications for Quantitative RT- PCR", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 2, 1998, pages 669 - 677, XP009141630
APPL ENVIRON MICROBIOL, vol. 62, no. 3, 1996, pages 1102 - 1106
PLOS ONE, vol. 5, no. 6, 2010, pages e11024
APPL ENVIRON MICROBIOL, vol. 64, no. 2, 1998, pages 669 - 677
BIOTECHNIQUES, vol. 31, no. 1, 2001, pages 81 - 86
SCIENCE, vol. 227, 1985, pages 1435 - 41
See also references of EP 4089104A4
CHANDLER, D. P. ET AL.: "Reverse Transcriptase (RT) Inhibition of PCR at Low Concentrations of Template and Its Implications for Quantitative RT- PCR", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 2, 1998, pages 669 - 677, XP009141630
APPL ENVIRON MICROBIOL, vol. 62, no. 3, 1996, pages 1102 - 1106
PLOS ONE, vol. 5, no. 6, 2010, pages e11024
APPL ENVIRON MICROBIOL, vol. 64, no. 2, 1998, pages 669 - 677
BIOTECHNIQUES, vol. 31, no. 1, 2001, pages 81 - 86
SCIENCE, vol. 227, 1985, pages 1435 - 41
See also references of EP 4089104A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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