Title:
METHOD FOR ANALYZING COMPONENTS OF IMMOBILIZED CELL
Document Type and Number:
WIPO Patent Application WO/2022/028535
Kind Code:
A1
Abstract:
Provided is a method for analyzing the components of an immobilized cell. The method comprises: packaging at most one immobilized cell and a particle containing a polynucleotide in a compartment together, wherein the polynucleotide contained in the particle contains at least one recognition sequence and at least one capture sequence, with the capture sequence being capable of directly or indirectly capturing components of the cell or a portion thereof; incubating the compartment under the conditions of decrosslinking the components of the cell or the portion thereof in the compartment and capturing the decrosslinked components of the cell or the portion thereof by means of the capture sequence; releasing the particle from the compartment; and analyzing the components of the immobilized cell or the ingredients thereof by means of analyzing a polynucleotide sequence on the released particle. Further provided are a kit for analyzing the components of an immobilized cell and a system for analyzing the components of an immobilized cell.
Inventors:
SHI WEIYANG (CN)
Application Number:
PCT/CN2021/110898
Publication Date:
February 10, 2022
Filing Date:
August 05, 2021
Export Citation:
Assignee:
10K GENOMICS (CN)
International Classes:
C12Q1/6806; C12N11/02; C12Q1/37; C12Q1/6809; C12Q1/686; C12Q1/6869; G01N33/50
Domestic Patent References:
| WO2019005763A1 | 2019-01-03 |
Foreign References:
| CN106574925A | 2017-04-19 | |||
| CN107463801A | 2017-12-12 |
Other References:
PHAN, H. V. ET AL.: "Fixed single-cell RNA sequencing for understanding virus infection and host response (Preprint)", BIORXIV, 17 September 2020 (2020-09-17), pages 1 - 34, XP055894845
JONATHAN ALLES, NIKOS KARAISKOS, SAMANTHA D. PRAKTIKNJO, STEFANIE GROSSWENDT, PHILIPP WAHLE, PIERRE-LOUIS RUFFAULT, SALAH AYOUB, L: "Cell fixation and preservation for droplet-based single-cell transcriptomics", BMC BIOLOGY, vol. 15, no. 1, 1 December 2017 (2017-12-01), XP055521622, DOI: 10.1186/s12915-017-0383-5
EVAN Z. MACOSKO, ANINDITA BASU, RAHUL SATIJA, JAMES NEMESH, KARTHIK SHEKHAR, MELISSA GOLDMAN, ITAY TIROSH, ALLISON R. BI: "Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets", CELL, vol. 161, no. 5, 1 May 2015 (2015-05-01), Amsterdam NL , pages 1202 - 1214, XP055586617, ISSN: 0092-8674, DOI: 10.1016/j.cell.2015.05.002
ELLIOT R THOMSEN, ET AL.: "Fixed single-cell transcriptomic characterization of human radial glial diversity", NATURE METHODS, vol. 13, no. 1, 16 November 2015 (2015-11-16), New York, pages 87 - 93, XP055454989, ISSN: 1548-7091, DOI: 10.1038/nmeth.3629
WANG, QUAN ET AL.: "Overview of the Technology of Single Cell Sequencing", CHINESE JOURNAL OF MEDICINAL GUIDE, vol. 22, no. 7, 31 July 2020 (2020-07-31), pages 433 - 439, XP055894854
JONATHAN ALLES, NIKOS KARAISKOS, SAMANTHA D. PRAKTIKNJO, STEFANIE GROSSWENDT, PHILIPP WAHLE, PIERRE-LOUIS RUFFAULT, SALAH AYOUB, L: "Cell fixation and preservation for droplet-based single-cell transcriptomics", BMC BIOLOGY, vol. 15, no. 1, 1 December 2017 (2017-12-01), XP055521622, DOI: 10.1186/s12915-017-0383-5
EVAN Z. MACOSKO, ANINDITA BASU, RAHUL SATIJA, JAMES NEMESH, KARTHIK SHEKHAR, MELISSA GOLDMAN, ITAY TIROSH, ALLISON R. BI: "Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets", CELL, vol. 161, no. 5, 1 May 2015 (2015-05-01), Amsterdam NL , pages 1202 - 1214, XP055586617, ISSN: 0092-8674, DOI: 10.1016/j.cell.2015.05.002
ELLIOT R THOMSEN, ET AL.: "Fixed single-cell transcriptomic characterization of human radial glial diversity", NATURE METHODS, vol. 13, no. 1, 16 November 2015 (2015-11-16), New York, pages 87 - 93, XP055454989, ISSN: 1548-7091, DOI: 10.1038/nmeth.3629
WANG, QUAN ET AL.: "Overview of the Technology of Single Cell Sequencing", CHINESE JOURNAL OF MEDICINAL GUIDE, vol. 22, no. 7, 31 July 2020 (2020-07-31), pages 433 - 439, XP055894854
Attorney, Agent or Firm:
SHANGHAI DIANSHI PARTNERS, P.C. (CN)
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