Title:
METHOD FOR ANALYZING NUCLEIC ACID DERIVED FROM SINGLE CELL
Document Type and Number:
WIPO Patent Application WO/2015/166768
Kind Code:
A1
Abstract:
Provided is a method for analyzing the configuration of a nucleic acid derived from a single cell with the use of a microplate provided with a plurality of reaction wells, wherein: one bead is placed in a reaction well of the microplate; a plurality of single-stranded oligonucleotide molecules are bound onto the bead; in the single-stranded oligonucleotide, a nucleic acid capture sequence is exposed in the 3'-terminal side and a barcode sequence is contained in the 5'-terminal side of the nucleic acid capture sequence; and the barcode sequence is a base sequence that differs from bead to bead.
Inventors:
HASHIMOTO SHIN-ICHI (JP)
KANEKO SHUICHI (JP)
MATSUSHIMA KOUJI (JP)
KANEKO SHUICHI (JP)
MATSUSHIMA KOUJI (JP)
Application Number:
PCT/JP2015/060841
Publication Date:
November 05, 2015
Filing Date:
April 07, 2015
Export Citation:
Assignee:
UNIV KANAZAWA NAT UNIV CORP (JP)
International Classes:
C12Q1/68; C12N15/09; C12N15/11; G01N33/543
Domestic Patent References:
| WO2012048341A1 | 2012-04-12 | |||
| WO2010087466A1 | 2010-08-05 |
Foreign References:
| JP2010110262A | 2010-05-20 | |||
| JP2014506788A | 2014-03-20 | |||
| JP2002503954A | 2002-02-05 | |||
| JP2011036256A | 2011-02-24 |
Other References:
MARGULIES MARCEL ET AL.: "Genome sequencing in microfabricated high-density picolitre reactors", NATURE, vol. 437, 2005, pages 376 - 380, XP002398505
Attorney, Agent or Firm:
SHOJI, Takashi et al. (JP)
Takashi Shoji (JP)
Takashi Shoji (JP)
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