Title:
METHOD FOR ANALYZING REACTIVE LOW-MOLECULAR-WEIGHT COMPOUND CONTAINED IN POLYOXYETHYLENE DERIVATIVE
Document Type and Number:
WIPO Patent Application WO/2023/286617
Kind Code:
A1
Abstract:
The present invention provides an analysis method in which a compound of formula (1) contained in a polyoxyethylene derivative is separated by means of reversed-phase chromatography, and the compound is subsequently detected by means of an ultraviolet-visible spectroscopic detector, the analysis method being characterized in that: ultraviolet light having a wavelength shorter than 200 nm is used as a detection wavelength; and an acidic substance that has a pKa of 4 or less and a maximum molar extinction coefficient of 20 M-1 cm-1 or less at 190 nm to 200 nm is used as an additive to a mobile phase for analysis.
Inventors:
KAMIYA MASAKI (JP)
SUZUKI AKIRA (JP)
YOSHIOKA HIROKI (JP)
SUZUKI AKIRA (JP)
YOSHIOKA HIROKI (JP)
Application Number:
PCT/JP2022/026213
Publication Date:
January 19, 2023
Filing Date:
June 30, 2022
Export Citation:
Assignee:
NOF CORP (JP)
International Classes:
B01J20/287; G01N30/26; G01N30/74; G01N30/88
Domestic Patent References:
| WO2010107520A1 | 2010-09-23 |
Foreign References:
| JP2005208050A | 2005-08-04 | |||
| JP2018172649A | 2018-11-08 | |||
| JP2021115693A | 2021-08-10 |
Other References:
WILCHEK MEIR, MIRON TALIA: "Limitations of A-Hydroxysuccinimide Esters in Affinity Chromatography and Protein Immobilization1'", BIOCHEMISTRY, vol. 26, no. 141, 1 January 1987 (1987-01-01), pages 2155 - 2161, XP093023921, ISSN: 0006-2960
GROSS H., BILK L.: "Zur reaktion von N-Hydroxysuccinimid mit dicyclohexylcarbodiimid", TETRAHEDRON, ELSEVIER SIENCE PUBLISHERS, AMSTERDAM, NL, vol. 24, no. 24, 1 January 1968 (1968-01-01), AMSTERDAM, NL , pages 6935 - 6939, XP093023922, ISSN: 0040-4020, DOI: 10.1016/S0040-4020(01)96807-3
TETRAHEDRON, vol. 24, no. 21994-89-8, 1968, pages 6935 - 6939
REACTIVE FUNCTIONAL POLYMERS, vol. 67, 2007, pages 529 - 539
GROSS H., BILK L.: "Zur reaktion von N-Hydroxysuccinimid mit dicyclohexylcarbodiimid", TETRAHEDRON, ELSEVIER SIENCE PUBLISHERS, AMSTERDAM, NL, vol. 24, no. 24, 1 January 1968 (1968-01-01), AMSTERDAM, NL , pages 6935 - 6939, XP093023922, ISSN: 0040-4020, DOI: 10.1016/S0040-4020(01)96807-3
TETRAHEDRON, vol. 24, no. 21994-89-8, 1968, pages 6935 - 6939
REACTIVE FUNCTIONAL POLYMERS, vol. 67, 2007, pages 529 - 539
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
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