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Title:
METHOD AND APPARATUS FOR DETERMINING THE EFFECT OF A DRUG ON CELLS
Document Type and Number:
WIPO Patent Application WO2001026609
Kind Code:
A3
Abstract:
A method is described for determining the effect of a drug or drugs on an attached culture of cells comprising (i) dissolving the phosphorescent compound, of known or predetermined quenching constant and lifetime at zero oxygen, in a culture medium at a selected temperature comprising an attached culture of test cells; (ii) introducing the drug(s), whose effect on the test cells is to be determined, into the culture medium; (iii) illuminating the culture medium with pulsed or modulated light at a level sufficient to cause the phosphorescent compound to emit measurable phosphorescence; (iv) measuring the emitted phosphorescent; and (v) calculating the phosphorescence lifetime and oxygen concentration gradient in the medium, thereby determining the effect of the drug on the respiration rate of the cells at the selected temperature. Apparatus for carrying out the method is also disclosed.

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Inventors:
WILSON DAVID F (US)
VINOGRADOV SERGEI A (US)
Application Number:
PCT/US2000/028481
Publication Date:
January 10, 2002
Filing Date:
October 13, 2000
Export Citation:
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Assignee:
UNIV PENNSYLVANIA (US)
WILSON DAVID F (US)
VINOGRADOV SERGEI A (US)
International Classes:
C12M1/34; G01N33/50; G01N33/52; (IPC1-7): G01J1/58; G01N21/64; G01T1/00
Foreign References:
US5792426A1998-08-11
US5830138A1998-11-03
US5501225A1996-03-26
Other References:
MOTTERLINI ET AL.: "Depression of endothelial and smooth muscle cell oxygen consumption by endotoxin", AMERICAN JOURNAL OF PHYSIOL., vol. 275, no. 168, September 1998 (1998-09-01), pages H776 - H782, XP002939132
INCE ET AL.: "Microcirculatory oxygenation and shunting in sepsis and shock", CIR. CARE. MED., vol. 27, no. 7, 1999, pages 1369 - 1377, XP002939133
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