Title:
METHOD AND APPARATUS FOR MEASURING NITRIC OXIDE PRODUCTION AND OXYGEN CONSUMPTION IN CULTURES OF ADHERENT CELLS
Document Type and Number:
WIPO Patent Application WO2003102124
Kind Code:
A3
Abstract:
An apparatus and method for measuring nitric oxide production and oxygen consumption in cultures of adherent cells continuously and without destroying the cells. The method involves flowing growth media through a tube having adherent cells are adhered to the inner surface thereof and then contacting the growth media with an NO or O2 sensor to detect the concentration of NO or O2 in the growth media.
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Inventors:
BAUMGARDNER JAMES E (US)
OTTO CYNTHIA M (US)
OTTO CYNTHIA M (US)
Application Number:
PCT/US2003/017373
Publication Date:
March 25, 2004
Filing Date:
June 02, 2003
Export Citation:
Assignee:
UNIV PENNSYLVANIA (US)
BAUMGARDNER JAMES E (US)
OTTO CYNTHIA M (US)
BAUMGARDNER JAMES E (US)
OTTO CYNTHIA M (US)
International Classes:
B01L5/00; C12M1/00; C12M1/04; C12M1/36; C12M3/00; C12Q1/00; C12Q1/02; C12Q1/26; G01N33/00; G01N33/50; (IPC1-7): G01N33/50
Foreign References:
| US6133567A | 2000-10-17 | |||
| US4439679A | 1984-03-27 | |||
| US6269679B1 | 2001-08-07 | |||
| US5434085A | 1995-07-18 | |||
| US6280604B1 | 2001-08-28 |
Other References:
BAUMGARDNER JAMES E ET AL: "In vitro intermittent hypoxia: Challenges for creating hypoxia in cell culture.", RESPIRATORY PHYSIOLOGY AND NEUROBIOLOGY, vol. 136, no. 2-3, 10 April 2003 (2003-04-10) - 16 July 2003 (2003-07-16), pages 131 - 139, XP009023879, ISSN: 1569-9048 (ISSN print)
HALLMAN T M ET AL: "Oxygen dependence of nitric oxide synthesis in raw 264.7 cells", FASEB JOURNAL, vol. 13, no. 4 PART 1, 12 March 1999 (1999-03-12), Annual Meeting of the Professional Research Scientists for Experimental Biology 99;Washington, D.C., USA; April 17-21, 1999, pages A104, XP009023884, ISSN: 0892-6638
HALLMAN T M ET AL: "Oxygen dependence of nitric oxide synthesis in raw 264.7 cells", FASEB JOURNAL, vol. 13, no. 4 PART 1, 12 March 1999 (1999-03-12), Annual Meeting of the Professional Research Scientists for Experimental Biology 99;Washington, D.C., USA; April 17-21, 1999, pages A104, XP009023884, ISSN: 0892-6638
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