Title:
METHOD AND BIOSENSOR FOR DETECTING ANTIGEN
Document Type and Number:
WIPO Patent Application WO/2000/042433
Kind Code:
A1
Abstract:
A sensor chip for surface plasmon resonance which has a thin metal film, an antibody against a substance with the steroid skeleton such as a steroid hormone, a sex hormone or an environmental hormone (excluding triazine compounds), and a fixation layer for fixing the antibody to the thin metal film; and a method for detecting an environmental hormone which involves the step of exposing the antibody in the above-mentioned sensor chip to a sample, the step of irradiating the thin metal film with light and the step of determining the intensity of the light reflected by the thin metal film. This method achieves a high sensitivity and an excellent selectivity.
Inventors:
KARUBE ISAO (JP)
NOMURA YOKO (JP)
NOMURA YOKO (JP)
Application Number:
PCT/JP1999/000066
Publication Date:
July 20, 2000
Filing Date:
January 12, 1999
Export Citation:
Assignee:
EBARA CORP (JP)
KARUBE ISAO (JP)
NOMURA YOKO (JP)
KARUBE ISAO (JP)
NOMURA YOKO (JP)
International Classes:
G01N33/543; (IPC1-7): G01N33/543
Foreign References:
| JPH01109262A | 1989-04-26 | |||
| JPS6314691A | 1988-01-21 | |||
| JPH04501605A | 1992-03-19 | |||
| JPH10267834A | 1998-10-09 |
Other References:
H. MORGAN & D. M. TAYLOR: "A Surface plasmon resonance immnosensor based on the streptavidin-biotin complex.", BIOSENSORS & BIOELECTRONICS, vol. 7, 1992, pages 405 - 410, XP002933655
DAKEYAMA F, ET AL.: "METHOD FOR MEASURING OF HORMONAL ACTIVITY BASED ON INTERACTION BETWEEN NUCLEAR RECEPTOR AND CO-ACTIVATOR", KANKYOU TOKISOKOROJII SYMPOSIUM, XX, XX, 12 May 1998 (1998-05-12), XX, pages 81, XP002933656
DAKEYAMA F, ET AL.: "METHOD FOR MEASURING OF HORMONAL ACTIVITY BASED ON INTERACTION BETWEEN NUCLEAR RECEPTOR AND CO-ACTIVATOR", KANKYOU TOKISOKOROJII SYMPOSIUM, XX, XX, 12 May 1998 (1998-05-12), XX, pages 81, XP002933656
Attorney, Agent or Firm:
Shamoto, Ichio (New Ohtemachi Building 2-1, Ohtemachi 2-chome Chiyoda-ku Tokyo, JP)
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