Title:
METHOD OF BREEDING YEAST
Document Type and Number:
WIPO Patent Application WO/2001/031000
Kind Code:
A1
Abstract:
A DNA construct consisting of: (1) a screening marker gene; (2) a proliferation inhibitory sequence which can be induced with galactose; (3) a pair of FRT sequences located in such a direction that (1) and (2) are sandwiched between them; and (4) a DNA which can be recombined with yeast chromosomal DNAs provided in both sides of (3); characterized in that the FRT sequences contain the following sequence: 5'-GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC-3'(SEQ ID NO:1) inverted repeat spacer inverted repeat sequence (1) sequence sequence (2) or a sequence substantially identical therewith and, in each of the pairing FRT sequences, one to six bases have been deleted from the distal side of the spacer sequence in the inverted repeat sequence in the distal side of the screening marker gene and the proliferation inhibitory sequence sandwiched between them; a method of transforming a yeast belonging to the genus Saccharomyces by using the same; a yeast belonging to the genus Saccharomyces transformed thereby; and a process for producing beer characterized by using this yeast belonging to the genus Saccharomyces.
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Inventors:
ASHIKARI TOSHIHIKO (JP)
OCHIAI MISA (JP)
OCHIAI MISA (JP)
Application Number:
PCT/JP2000/007491
Publication Date:
May 03, 2001
Filing Date:
October 26, 2000
Export Citation:
Assignee:
SUNTORY LTD (JP)
ASHIKARI TOSHIHIKO (JP)
OCHIAI MISA (JP)
ASHIKARI TOSHIHIKO (JP)
OCHIAI MISA (JP)
International Classes:
C12C11/02; C12N1/19; C12N15/81; C12N15/09; C12R1/85; (IPC1-7): C12N15/11; C12N1/19; C12C11/02
Foreign References:
| EP0814165A2 | 1997-12-29 | |||
| EP0699748A2 | 1996-03-06 |
Other References:
STORICI F. ET AL.: "A 2-microm DNA-based marker recycling system for multiple gene disruption in the yeast Saccharomyces cerevisiae", YEAST, vol. 15, no. 4, March 1999 (1999-03-01), pages 271 - 283, XP002937065
KAWAHATA M.: "A positive selection for plasmid loss in Saccharomyces cerevisiae using galactose-inducible growth inhibitory sequences", YEAST, vol. 15, no. 1, January 1999 (1999-01-01), pages 1 - 10, XP002937066
KAWAHATA M.: "A positive selection for plasmid loss in Saccharomyces cerevisiae using galactose-inducible growth inhibitory sequences", YEAST, vol. 15, no. 1, January 1999 (1999-01-01), pages 1 - 10, XP002937066
Attorney, Agent or Firm:
Shamoto, Ichio (New Ohtemachi Building 2-1 Ohtemachi 2-chome Chiyoda-ku, Tokyo, JP)
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