Title:
METHOD FOR CAUSING GENE TO BE EXPRESSED IN C/EBPα-, C/EBPβ-, C/EBPδ- OR p53-DEPENDENT MANNER USING PARTIAL SEQUENCE OF HUMAN decorin PROMOTER, AND METHOD FOR INHIBITING OR DETERMINING TRANSCRIPTION INDUCTION ABILITY OF C/EBPα, C/EBPβ, C/EBPδ OR p53
Document Type and Number:
WIPO Patent Application WO/2010/079802
Kind Code:
A1
Abstract:
Disclosed are a method for causing a gene to be expressed in a C/EBPα-, C/EBPβ-, C/EBPδ- or p53-dependent manner and a method for inhibiting or determining a transcription induction ability of C/EBPα, C/EBPβ, C/EBPδ or p53, in each of which a partial sequence of human decorin promoter is used. Also disclosed is DNA for use in the above-mentioned methods. The DNA comprises a nucleotide sequence depicted in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3, a nucleotide sequence produced by substituting or deleting one or more nucleotides in the nucleotide sequence depicted in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3, or a nucleotide sequence capable of hybridizing with any one of the above-mentioned nucleotide sequences under stringent conditions, and has a function to regulate the transcription induction ability of C/EBPα, C/EBPβ, C/EBPδ or p53.
Inventors:
HIWASA TAKAKI (JP)
TAKIGUCHI MASAKI (JP)
IWASE KATSURO (JP)
TAKIGUCHI MASAKI (JP)
IWASE KATSURO (JP)
Application Number:
PCT/JP2010/050095
Publication Date:
July 15, 2010
Filing Date:
January 07, 2010
Export Citation:
Assignee:
UNIV CHIBA NAT UNIV CORP (JP)
HIWASA TAKAKI (JP)
TAKIGUCHI MASAKI (JP)
IWASE KATSURO (JP)
HIWASA TAKAKI (JP)
TAKIGUCHI MASAKI (JP)
IWASE KATSURO (JP)
International Classes:
C12N15/09; C12Q1/68
Other References:
CORTES-CANTELI M ET AL.: "Microarray analysis supports a role for ccaat/enhancer-binding protein-beta in brain injury", J. BIOL. CHEM., vol. 279, no. 14, 2004, pages 14409 - 14417
NAMIKO KUROIWA ET AL.: "C/EBP Family no Saibo Zoshoku Yokuseino: Antei Donyu Kabu o Mochiita Kaiseki", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 73, no. 8, 2001, pages 716
HE F. ET AL.: "Upregulation of decorin by FXR in vascular smooth muscle cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 372, no. 4, 2008, pages 746 - 751
MAUVIEL A ET AL.: "Identification of a bimodal regulatory element encompassing a canonical AP-1 binding site in the proximal promoter region of the human decorin gene", J. BIOL. CHEM., vol. 271, no. 40, 1996, pages 24824 - 24829
TIMCHENKO NA ET AL.: "CCAAT/enhancer-binding protein alpha (C/EBP alpha) inhibits cell proliferation through the p21 (WAF-1/CIP-1/ SDI-1) protein", GENES DEV., vol. 10, no. 7, 1996, pages 804 - 815
TAKIGUCHI M.: "The C/EBP family of transcription factors in the liver and other organs", INT. J. EXP. PATHOL., vol. 79, no. 6, 1998, pages 369 - 391
SCHAEFER L ET AL.: "Biological functions of the small leucine-rich proteoglycans: from genetics to signal transduction", J. BIOL. CHEM., vol. 283, no. 31, 2008, pages 21305 - 20309
IOZZO R.V. ET AL: "Altered proteoglycan gene expression and the tumor stroma", EXPERIENTIA, vol. 49, no. 5, 1993, pages 447 - 455
SANTRA M. ET AL: "Structural and functional characterization of the human decorin gene promoter", J. BIOL. CHEM., vol. 269, no. 1, 1994, pages 579 - 587
DEMOOR-FOSSARD M ET AL.: "A composite element binding the vitamin D receptor and the retinoic X receptor alpha mediates the transforming growth factor-beta inhibition of decorin gene expression in articular chondrocytes", J. BIOL. CHEM., vol. 276, no. 40, 2001, pages 36983 - 36992
NAMIKO KUROIWA ET AL.: "C/EBP Family no Saibo Zoshoku Yokuseino: Antei Donyu Kabu o Mochiita Kaiseki", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 73, no. 8, 2001, pages 716
HE F. ET AL.: "Upregulation of decorin by FXR in vascular smooth muscle cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 372, no. 4, 2008, pages 746 - 751
MAUVIEL A ET AL.: "Identification of a bimodal regulatory element encompassing a canonical AP-1 binding site in the proximal promoter region of the human decorin gene", J. BIOL. CHEM., vol. 271, no. 40, 1996, pages 24824 - 24829
TIMCHENKO NA ET AL.: "CCAAT/enhancer-binding protein alpha (C/EBP alpha) inhibits cell proliferation through the p21 (WAF-1/CIP-1/ SDI-1) protein", GENES DEV., vol. 10, no. 7, 1996, pages 804 - 815
TAKIGUCHI M.: "The C/EBP family of transcription factors in the liver and other organs", INT. J. EXP. PATHOL., vol. 79, no. 6, 1998, pages 369 - 391
SCHAEFER L ET AL.: "Biological functions of the small leucine-rich proteoglycans: from genetics to signal transduction", J. BIOL. CHEM., vol. 283, no. 31, 2008, pages 21305 - 20309
IOZZO R.V. ET AL: "Altered proteoglycan gene expression and the tumor stroma", EXPERIENTIA, vol. 49, no. 5, 1993, pages 447 - 455
SANTRA M. ET AL: "Structural and functional characterization of the human decorin gene promoter", J. BIOL. CHEM., vol. 269, no. 1, 1994, pages 579 - 587
DEMOOR-FOSSARD M ET AL.: "A composite element binding the vitamin D receptor and the retinoic X receptor alpha mediates the transforming growth factor-beta inhibition of decorin gene expression in articular chondrocytes", J. BIOL. CHEM., vol. 276, no. 40, 2001, pages 36983 - 36992
Attorney, Agent or Firm:
TAKAHASHI MASAYOSHI (JP)
Masayoshi Takahashi (JP)
Masayoshi Takahashi (JP)
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