METHOD FOR CELL-BASED ASSAY TO DETECT MOG-IGG IN PATIENTS WITH CENTRAL NERVOUS SYSTEM INFLAMMATORY DISEASES

WIPO Patent Application WO/2021/162325

A1

The present invention relates to: a method for detecting myelin oligodendrocyte glycoprotein (MOG)-IgG comprising the steps of: (a) cloning a nucleic acid encoding MOG into a fluorescent protein expression vector; (b) transfecting a cell with the expression vector cloned with MOG; (c) selecting a stable transfected cell line by culturing the transfected cell in a medium containing a selection drug; (d) incubating the selected cell line and a sample expected to contain MOG-IgG together; (e) forming MOG-IgG and a secondary antibody conjugate by adding a fluorescence-conjugated secondary antibody to the incubated sample; and (f) detecting or analyzing fluorescence from the MOG-IgG and the secondary antibody conjugate; a method of providing information for prediction or diagnosis of MOG-IgG-related central nervous system inflammatory diseases; a composition for diagnosis of MOG-IgG-related central nervous system inflammatory diseases; and a method of diagnosing MOG-IgG-related central nervous system inflammatory diseases. The present invention confirms that there is a strong positive correlation between CBA-IF and CBA-FACS results when using fluorescence-conjugated IgG (H+L) as a secondary antibody specifically binding to MOG-IgG, and a high matching rate of about 98% is shown even compared with the existing IgG1 (Oxford) CBA-IF. In particular, the method of the present invention does not require repetitive transfection by constructing a stable cell line expressing MOG, and is advantageous in that there is no cross reaction with secondary antibody IgM, so that a more clear fluorescence signal having a high specificity can be obtained.

KIM HO JIN (KR)
CHOI KYUNGHO (KR)

PCT/KR2021/001402

August 19, 2021

February 03, 2021

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NAT CANCER CT (KR)

G01N33/68; C07K16/28; C12N5/071; G01N33/53

KR20190097067A	2019-08-20
KR102177224B1	2020-11-11

KIM YESEUL, HYUN JAE-WON, WOODHALL MARK R, OH YU-MI, LEE JI-EUN, JUNG JI YUN, KIM SO YEON, LEE MIN YOUNG, KIM SU-HYUN, KIM WOOJUN,: "Refining cell-based assay to detect MOG-IgG in patients with central nervous system inflammatory diseases", MULTIPLE SCLEROSIS AND RELATED DISORDERS, vol. 40, 1 May 2020 (2020-05-01), pages 1 - 9, XP055835782, ISSN: 2211-0348, DOI: 10.1016/j.msard.2020.101939
YING PENG , LEI LIU , YUANCHU ZHENG , ZHIXIN QIAO , KAI FENG , JIAWEI WANG: "Diagnostic implications of MOG/AQP4 antibodies in recurrent optic neuritis", EXPERIMENTAL AND THERAPEUTIC MEDICINE, vol. 16, no. 2, 8 June 2018 (2018-06-08), pages 950 - 958, XP055835786, ISSN: 1792-0981, DOI: 10.3892/etm.2018.6273
KIM YESEUL, KIM GAYOUNG, KONG BYUNG SOO, LEE JI-EUN, OH YU-MI, HYUN JAE-WON, KIM SU-HYUN, JOUNG AERAN, KIM BYOUNG JOON, CHOI KYUNG: "Large-Scale in-House Cell-Based Assay for Evaluating the Serostatus in Patients with Neuromyelitis Optica Spectrum Disorder Based on New Diagnostic Criteria", JOURNAL OF CLINICAL NEUROLOGY, vol. 13, no. 2, 1 January 2017 (2017-01-01), pages 175 - 180, XP055835792, ISSN: 1738-6586, DOI: 10.3988/jcn.2017.13.2.175

YOON & YANG (IP) LLC (KR)

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