Title:
METHOD OF CELL FRACTIONATION AND SUBSTRATE TO BE USED FOR THE METHOD
Document Type and Number:
WIPO Patent Application WO/2007/055178
Kind Code:
A1
Abstract:
In a method of cell fractionation according to the invention, by using a ligand-presenting substrate in which a ligand capable of temporarily binding to a receptor present on the surface of a cell desired to be fractionated is immobilized via a graft chain, cells are rotationally transferred on the surface of the ligand-presenting substrate and a difference in the transfer rate caused by the binding degree is utilized. This achieves a method of fractionating a cell presenting a specific receptor on the cell surface from a group of cells including the cell, in which the cell having a receptor density within a predetermined range can be fractionated efficiently and highly accurately and the cells having respective densities can be continuously fractionated from the group of cells depending on the receptor density.
Inventors:
YAMAOKA TETSUJI
MAHARA ATSUSHI
KITAMURA SOICHIRO
MAHARA ATSUSHI
KITAMURA SOICHIRO
Application Number:
PCT/JP2006/322119
Publication Date:
May 18, 2007
Filing Date:
November 06, 2006
Export Citation:
Assignee:
JAPAN HEALTH SCIENCE FOUND (JP)
YAMAOKA TETSUJI
MAHARA ATSUSHI
KITAMURA SOICHIRO
YAMAOKA TETSUJI
MAHARA ATSUSHI
KITAMURA SOICHIRO
International Classes:
C07K17/00; C12M1/00; C12N5/07; C12N5/074; C12N5/0789
Domestic Patent References:
| WO2004027050A1 | 2004-04-01 |
Foreign References:
| JP2005511074A | 2005-04-28 | |||
| JPH07136505A | 1995-05-30 |
Other References:
UBAHARA A. ET AL.: "Kotai Koteika Column System ni yoru Kansaibo Bunri", THE SOCIETY OF POLYMER SCIENCE, JAPAN IRYO KOBUNSHI SYMPOSIUM KOEN YOSHISHU, vol. 35TH, 1 August 2006 (2006-08-01), pages 47 - 48
UMAHARA A. ET AL.: "Ligand Koteika Column ni yoru Kansaibo Bunriho no Kaihatsu", THE SOCIETY OF POLYMER SCIENCE, JAPAN NENJI TAIKAI YOKOSHU (CD-ROM), vol. 55, no. 1, 10 May 2006 (2006-05-10), pages 3JI5
UMAHARA A. ET AL.: "Saibo Bunri Column ni yoru Kinosei Kansaibo no Subpopulation Bunri to Kino Hyoka", THE JAPANESE JOURNAL OF ARTIFICIAL ORGANS, vol. 35, no. 2, 1 October 2006 (2006-10-01), pages S-126
UMAHARA A. ET AL.: "Ligand Koteika Column ni yoru Kansaibo Bunriho no Kaihatsu", THE SOCIETY OF POLYMER SCIENCE, JAPAN NENJI TAIKAI YOKOSHU (CD-ROM), vol. 55, no. 1, 10 May 2006 (2006-05-10), pages 3JI5
UMAHARA A. ET AL.: "Saibo Bunri Column ni yoru Kinosei Kansaibo no Subpopulation Bunri to Kino Hyoka", THE JAPANESE JOURNAL OF ARTIFICIAL ORGANS, vol. 35, no. 2, 1 October 2006 (2006-10-01), pages S-126
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (2-6 Tenjinbashi 2-chome, Kita, Kita-ku, Osaka-sh, Osaka 41, JP)
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