Title:
METHOD OF CHANGING FLUORESCENCE WAVELENGTH OF FLUOROPROTEIN
Document Type and Number:
WIPO Patent Application WO/2007/086473
Kind Code:
A1
Abstract:
It is intended to provide a method which comprises specifying a structural factor determining the fluorescence wavelength in the three-dimensional structure of a GFP-like fluoroprotein originating in Copepod and modifying an amino acid residue participating in the structural factor so as to change the fluorescence wavelength while sustaining the recombination expression efficiency thereof; and a modified fluoroprotein that is obtained by applying this method. For example, in a GFP-like protein originating in Chiridius poppei, His52 in the α-helix type secondary structure: PFLLSHCMGYGFYHF(α1 47-61) including a fluorescent group GYG is substituted by an aromatic amino acid selected from among Phe, Tyr and Trp to cause a color shift of the fluorescence peak wavelength toward red; or substituted by Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln to cause a color shift of the fluorescence peak wavelength toward blue.
Inventors:
SUTO KYOKO (JP)
TAKENAKA HIROMI (JP)
TAKENAKA YASUHIRO (JP)
TAKENAKA HIROMI (JP)
TAKENAKA YASUHIRO (JP)
Application Number:
PCT/JP2007/051188
Publication Date:
August 02, 2007
Filing Date:
January 25, 2007
Export Citation:
Assignee:
NEC SOFTWARE LTD (JP)
SUTO KYOKO (JP)
TAKENAKA HIROMI (JP)
TAKENAKA YASUHIRO (JP)
SUTO KYOKO (JP)
TAKENAKA HIROMI (JP)
TAKENAKA YASUHIRO (JP)
International Classes:
C12N15/00; C07K14/435; C12N15/09
Domestic Patent References:
| WO2005095599A1 | 2005-10-13 | |||
| WO2004058973A1 | 2004-07-15 |
Other References:
MIYAWAKI A. ET AL: "Mechanisms of protein fluorophore formation and engineering", CURR. OPIN. CHEM. BIOL., vol. 7, no. 5, 2003, pages 557 - 562, XP003016061
MATZ M.V. ET AL: "Fluorescent proteins from nonbioluminescent Anthozoa species", NAT. BIOTECHNOL., vol. 17, no. 10, 1999, pages 969 - 973, XP000882891
CUBITT A.B. ET AL: "Understanding, improving and using green fluorescent proteins", TRENDS BIOCHEM. SCI., vol. 20, no. 11, 1995, pages 448 - 455, XP000606919
JAIN R.K. ET AL: "Local complexity of amino acid interactions in a protein core", PROC. NATL. ACAD. SCI. U.S.A., vol. 101, no. 1, 2004, pages 111 - 116, XP003016062
See also references of EP 1988161A4
MATZ M.V. ET AL: "Fluorescent proteins from nonbioluminescent Anthozoa species", NAT. BIOTECHNOL., vol. 17, no. 10, 1999, pages 969 - 973, XP000882891
CUBITT A.B. ET AL: "Understanding, improving and using green fluorescent proteins", TRENDS BIOCHEM. SCI., vol. 20, no. 11, 1995, pages 448 - 455, XP000606919
JAIN R.K. ET AL: "Local complexity of amino acid interactions in a protein core", PROC. NATL. ACAD. SCI. U.S.A., vol. 101, no. 1, 2004, pages 111 - 116, XP003016062
See also references of EP 1988161A4
Attorney, Agent or Firm:
MIYAZAKI, Teruo et al. (16th Kowa Bldg. 9-1, Akasaka 1-chom, Minato-ku Tokyo 52, JP)
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