METHOD FOR CO-PRODUCTION OF YEAST GLUCAN, MANNOSE PROTEIN AND YEAST EXTRACT USING BETA-1,6-GLUCANASE

WIPO Patent Application WO/2020/192496

A1

Disclosed is a method for co-production of yeast glucan, mannose protein and yeast extract using beta-1,6-glucanase. The method comprises the following steps: taking yeast cells as a raw material, subjecting the yeast cells to a high-temperature deactivation treatment, performing centrifugation and drying the supernatant to obtain a yeast extract; performing enzymolysis on yeast cell walls by means of beta-1,6-glucanase, centrifuging same to obtain an enzymatic hydrolysate supernatant and an enzymolysis precipitate, and subjecting the enzymatic hydrolysate supernatant to water extraction, alcohol precipitation, centrifugal separation and drying to obtain a mannose protein; after the recovery of ethanol, subjecting residual liquid to concentration and spray drying to obtain a yeast extract; and subjecting the enzymolysis precipitate to degreasing, protease treatment and spray drying to obtain yeast glucan. The yeast glucan and the mannose protein prepared in the present invention are high in purity and yield, and the preparation processes therefor are low in cost and low in environmental pollution due to the avoidance of the consumption of strong acid and strong base agents.

CUI ZHONGLI (CN)
LI ZHOUKUN (CN)
QIAO YAN (CN)
YE XIANFENG (CN)
CAO HUI (CN)
FANG XIAODONG (CN)

PCT/CN2020/079724

October 01, 2020

March 17, 2020

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UNIV NANJING AGRICULTURAL (CN)

C07K14/395; C07K1/14; C07K1/34; C08B37/02; C12N1/06; C12R1/865

CN1940084A	2007-04-04
BR102015016211A2	2016-11-29
RU2504384C2	2014-01-20
CN104862355A	2015-08-26
CN102351943A	2012-02-15
CN109912700A	2019-06-21

NANJING TIANHUA PATENT AGENT CO., LTD. (CN)

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