Title:
METHOD FOR CONSTRUCTING ERGOTHIONEINE-PRODUCING STRAIN
Document Type and Number:
WIPO Patent Application WO/2021/185039
Kind Code:
A1
Abstract:
Provided are a Rhodotorula toruloides engineering bacteria and a construction method therefor. The Rhodotorula toruloides engineering bacteria can express exogenous egt1 enzymes, so as to improve the capacity for producing ergothioneine. After fermentation, the yield of ergothioneine is close to 1.5 g/L, and the genetic characteristics of the engineering bacteria are stable.
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Inventors:
FAN WENCHAO (CN)
GAO SHULIANG (CN)
WANG JINGANG (CN)
REN LIANG (CN)
YU XIANG (CN)
GAO SHULIANG (CN)
WANG JINGANG (CN)
REN LIANG (CN)
YU XIANG (CN)
Application Number:
PCT/CN2021/077815
Publication Date:
September 23, 2021
Filing Date:
February 25, 2021
Export Citation:
Assignee:
ZHEJIANG HUARUI BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/81; C12N1/19; C12N15/53; C12N15/66; C12P13/04; C12R1/645
Domestic Patent References:
| WO2016104437A1 | 2016-06-30 |
Foreign References:
| CN111363760A | 2020-07-03 | |||
| CN111534535A | 2020-08-14 | |||
| CN110551697A | 2019-12-10 | |||
| CN110607286A | 2019-12-24 | |||
| CN110358719A | 2019-10-22 |
Other References:
STEVEN A. VAN DER HOEK, BEHROOZ DARBANI, KAROLINA E. ZUGAJ, BALA KRISHNA PRABHALA, MATHIAS BERNFRIED BIRON, MILICA RANDELOVIC, JAC: "Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, XP055705666, DOI: 10.3389/fbioe.2019.00262
FUJITANI YOSHIKO; ALAMGIR KABIR MD; TANI AKIO: "Ergothioneine production usingMethylobacteriumspecies, yeast, and fungi", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 126, no. 6, 14 June 2018 (2018-06-14), NL, pages 715 - 722, XP085523515, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2018.05.021
DATABASE NUCLEOTIDE 11 October 2017 (2017-10-11), ANONYMOUS: "Neurospora crassa OR74A DUF323 domain-containing protein (egt-1), mRNA", XP055851673, retrieved from NCBI Database accession no. XM_951231
FUJITANI YOSHIKO; ALAMGIR KABIR MD; TANI AKIO: "Ergothioneine production usingMethylobacteriumspecies, yeast, and fungi", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 126, no. 6, 14 June 2018 (2018-06-14), NL, pages 715 - 722, XP085523515, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2018.05.021
DATABASE NUCLEOTIDE 11 October 2017 (2017-10-11), ANONYMOUS: "Neurospora crassa OR74A DUF323 domain-containing protein (egt-1), mRNA", XP055851673, retrieved from NCBI Database accession no. XM_951231
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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