Title:
METHOD FOR CONSTRUCTING THREONINE-PRODUCING ENGINEERED BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2023/142861
Kind Code:
A9
Abstract:
The present invention provides a method for constructing a threonine-producing engineered bacterium. According to the present invention, a 2-formyl-citrate synthase 1-inactivated strain (corynebacterium) is applied to the production of threonine, and the yield of threonine produced by the 2-formyl-citrate synthase 1-inactivated strain is increased by about 42% compared with that produced by an unengineered strain. When the application of the 2-formyl-citrate synthase 1-inactivated strain is further combined with enhanced expression of at least one of aspartate aminotransferase, aspartate kinase, homoserine dehydrogenase, threonine synthase, NAD kinase, fructose-1,6-bisphosphatase 2 and the like in the threonine synthesis pathway, the yield of threonine is improved. The method provides a new way for large-scale production of threonine and has high application value.
Inventors:
KANG PEI (CN)
GONG WEIBO (CN)
HE JUN (CN)
LI YAN (CN)
GONG WEIBO (CN)
HE JUN (CN)
LI YAN (CN)
Application Number:
PCT/CN2022/143105
Publication Date:
September 21, 2023
Filing Date:
December 29, 2022
Export Citation:
Assignee:
LANGFANG MEIHUA BIOTECHNOLOGY DEV CO LTD (CN)
International Classes:
C12N1/21; C12N15/63; C12N15/77; C12P13/08; C12R1/15
Attorney, Agent or Firm:
BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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