Title:
METHOD FOR CONTROLLING DNA CLEAVAGE ACTIVITY OF Cas9 NUCLEASE
Document Type and Number:
WIPO Patent Application WO/2021/065155
Kind Code:
A1
Abstract:
Provided is a method for controlling the DNA cleavage activity of Cas9 nuclease, the method comprising (1) a step for bringing a CRISPR-Cas9 ribonucleoprotein complex into contact with a target DNA, wherein the complex comprises a guide RNA and an inactivated Cas9 nuclease containing a point mutation at the histidine residue corresponding to the position 840 according to numbering based on the amino acid sequence of a Streptococcus pyogenes-derived Cas9 nuclease; and then (2) a step for bringing the complex into contact with imidazole or a derivative thereof.
Inventors:
KATO YOSHIO (JP)
FURUHATA YUUICHI (JP)
FURUHATA YUUICHI (JP)
Application Number:
PCT/JP2020/027786
Publication Date:
April 08, 2021
Filing Date:
July 17, 2020
Export Citation:
Assignee:
AIST (JP)
International Classes:
C12N15/09; C12N9/16
Foreign References:
| JP2015527889A | 2015-09-24 | |||
| JP2016519652A | 2016-07-07 |
Other References:
LEE, H. Y. ET AL.: "RNA-protein analysis using a conditional CRISPR nuclease", PNAS, vol. 110, no. 14, 2013, pages 5416 - 5421, XP055282781, DOI: 10.1073/pnas.1302807110
JESSER, RABEA, BEHLER JULIANE, BENDA CHRISTIAN, REIMANN VIKTORIA, HESS WOLFGANG R.: "Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp.PCC 6803", RNA BIOLOGY, vol. 16, no. 4, 2019, pages 481 - 491, XP055813541, DOI: 10.1080/15476286.2018.1447742
JESSER, RABEA, BEHLER JULIANE, BENDA CHRISTIAN, REIMANN VIKTORIA, HESS WOLFGANG R.: "Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp.PCC 6803", RNA BIOLOGY, vol. 16, no. 4, 2019, pages 481 - 491, XP055813541, DOI: 10.1080/15476286.2018.1447742
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
Download PDF:
Previous Patent: TERMINAL AND COMMUNICATION METHOD
Next Patent: HEAT SOURCE UNIT AND REFRIGERATION DEVICE
Next Patent: HEAT SOURCE UNIT AND REFRIGERATION DEVICE