Title:
METHOD FOR CREATING DETERMINATE GROWTH PLANT TYPE COTTON
Document Type and Number:
WIPO Patent Application WO/2020/135243
Kind Code:
A1
Abstract:
Provided is a method for rapidly creating, by means of a fixed-point gene editing technique, a new cotton material, the stems and fruit spurs of which show determinate growth. According to the method, a plant type regulation and control candidate gene GhLSGZ is knocked out by means of a fixed-point gene editing technique so as to obtain a Ghlsgz mutant with determinate growth habits, wherein the mutant can be used for improving the plant type of the cotton, thereby obtaining a new cotton strain with a superior plant type. Transgenic elements can be removed by means of descendant separation of the obtained Ghlsgz mutant. Also provided is a fixed-point knockout mutant gene of the GhLSGZ gene, a functional target sequence for knocking out the GhLSGZ gene and a complementary primer thereof, a fixed-point knockout vector for knocking out the GhLSGZ gene, a fixed-point knockout method for the GhLSGZ gene of a zero-type fruit spur gene and a method for regulating and controlling the plant type and maturity period of the cotton.
Inventors:
CHEN WEI (CN)
ZHANG YONGSHAN (CN)
YAO JINBO (CN)
LI YAN (CN)
FANG SHENGTAO (CN)
ZHANG YONGSHAN (CN)
YAO JINBO (CN)
LI YAN (CN)
FANG SHENGTAO (CN)
Application Number:
PCT/CN2019/126867
Publication Date:
July 02, 2020
Filing Date:
December 20, 2019
Export Citation:
Assignee:
INSTITUTE OF COTTON RES CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C12N15/29; A01H1/02; A01H5/00; A01H6/60; C12N5/10; C12N15/113; C12N15/82
Domestic Patent References:
| WO2017009847A1 | 2017-01-19 |
Foreign References:
| CN105037518A | 2015-11-11 | |||
| EP2647646A1 | 2013-10-09 |
Other References:
DATABASE Nucleotide 9 August 2016 (2016-08-09), "PREDICTED: Gossypium arboreum CEN-like protein 2 (LOC108486309), trans", XP055723664, retrieved from NCBI Database accession no. XM_017790298
DATABASE Nucleotide 18 May 2016 (2016-05-18), "PREDICTED: Gossypium hirsutum CEN-like protein 2 (LOC107956523), mRNA", XP055723666, retrieved from NCBI Database accession no. XM_016892150
ARGIRIOU , A.: "Characterization and expression analysis of TERMINAL FLOWER1 homologs from cultivated alloteraploid cotton (Gossypium hirsutum) and its diploid progenitors", JOURNAL OF PLANT PHYSIOLOGY, vol. 165, no. 15, 2 January 2018 (2018-01-02), pages 1636 - 1646, XP025406788, ISSN: 0176-1617
DATABASE Nucleotide 18 May 2016 (2016-05-18), "PREDICTED: Gossypium hirsutum CEN-like protein 2 (LOC107956523), mRNA", XP055723666, retrieved from NCBI Database accession no. XM_016892150
ARGIRIOU , A.: "Characterization and expression analysis of TERMINAL FLOWER1 homologs from cultivated alloteraploid cotton (Gossypium hirsutum) and its diploid progenitors", JOURNAL OF PLANT PHYSIOLOGY, vol. 165, no. 15, 2 January 2018 (2018-01-02), pages 1636 - 1646, XP025406788, ISSN: 0176-1617
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
Download PDF: