Title:
METHOD OF CRYOPRESERVATION OF TISSUES OR ORGANS OTHER THAN A BLOOD VESSEL BY VITRIFICATION
Document Type and Number:
WIPO Patent Application WO2002032225
Kind Code:
A3
Abstract:
A method for vitrification of a tissue or organ other than a blood vessel includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than - 15 DEG C to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5-100 DEG C per minute to a temperature between - 80 DEG C and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30 DEG C per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20-40 DEG C per minute to a temperature between - 80 DEG C and the glass transition temperature; further warming the tissue or organ at a rate of from 200-300 DEG C per minute to a temperature above - 75 DEG C; and reducing the concentration of the cryoprotectant.
Inventors:
KHIRABADI BIJAN S
SONG YING C
BROCKBANK KELVIN G M
SONG YING C
BROCKBANK KELVIN G M
Application Number:
PCT/US2001/032415
Publication Date:
January 09, 2003
Filing Date:
October 18, 2001
Export Citation:
Assignee:
ORGAN RECOVERY SYSTEMS (US)
International Classes:
A01N1/02; (IPC1-7): A01N1/02
Domestic Patent References:
| WO1996005727A1 | 1996-02-29 | |||
| WO2000060935A1 | 2000-10-19 | |||
| WO2001078504A2 | 2001-10-25 |
Foreign References:
| US4559298A | 1985-12-17 |
Other References:
BOURNE W M ET AL: "HUMAN CORNEAL STUDIES WITH A VITRIFICATION SOLUTION CONTAINING DIMETHYL SULFOXIDE, FORMAMIDE, AND 1,2-PROPANEDIOL", CRYOBIOLOGY, ACADEMIC PRESS INC, US, vol. 31, no. 6, 1994, pages 522 - 530, XP000917091, ISSN: 0011-2240
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, PEGG DAVID E ET AL: "Fractures in cryopreserved elastic arteries.", XP002203120, Database accession no. PREV199799558143
ARMITAGE W J: "SURVIVAL OF CORNEAL ENDOTHELIUM FOLLOWING EXPOSURE TO A VITRIFICATION SOLUTION", CRYOBIOLOGY, ACADEMIC PRESS INC, US, vol. 26, no. 4, 1989, pages 318 - 327, XP000917067, ISSN: 0011-2240
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, PEGG DAVID E ET AL: "Fractures in cryopreserved elastic arteries.", XP002203120, Database accession no. PREV199799558143
ARMITAGE W J: "SURVIVAL OF CORNEAL ENDOTHELIUM FOLLOWING EXPOSURE TO A VITRIFICATION SOLUTION", CRYOBIOLOGY, ACADEMIC PRESS INC, US, vol. 26, no. 4, 1989, pages 318 - 327, XP000917067, ISSN: 0011-2240
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