Title:
METHOD FOR CULTIVATING LILIES CONTAINING DELPHININ IN THE PETALS THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/036290
Kind Code:
A1
Abstract:
Provided is a method for cultivating lilies containing a blue pigment (delphinidin) in the petals thereof by introducing a foreign gene into lilies. The method pertaining to the present invention is a method for cultivating lilies containing delphinidin in the petals thereof, including the following steps: introducing, into a lily, a F3'5'H gene derived from a campanula and comprising a base sequence encoding a peptide having flavonoid 3', 5'-hydroxylase (F3'5'H) activity, such as a base sequence represented by SEQ ID NO: 1 or SEQ ID NO: 11; while also introducing a F3'H gene fragment derived from a lily and comprising a base sequence encoding a peptide having flavonoid 3'-hydroxylase (F3'H) activity, such as a base sequence represented by SEQ ID NO:3 or SEQ ID NO:16; and inhibiting the expression of endogenous F3'H expression, which acts on cyanidin synthesis in lily petals, while the F3'5'H gene that has been introduced acts to cause the synthesis of delphinidin.
More Like This:
Inventors:
TANAKA YOSHIKAZU (JP)
NAKAMURA NORIKO (JP)
KOBAYASHI HITOSHI (JP)
OKUHARA HIROAKI (JP)
KONDO MASAYOSHI (JP)
KOIKE YOSUKE (JP)
HOSHI YOSUKE (JP)
NOMIZU TOSHIKAZU (JP)
NAKAMURA NORIKO (JP)
KOBAYASHI HITOSHI (JP)
OKUHARA HIROAKI (JP)
KONDO MASAYOSHI (JP)
KOIKE YOSUKE (JP)
HOSHI YOSUKE (JP)
NOMIZU TOSHIKAZU (JP)
Application Number:
PCT/JP2011/071271
Publication Date:
March 22, 2012
Filing Date:
September 16, 2011
Export Citation:
Assignee:
SUNTORY HOLDINGS LTD (JP)
NIIGATA PREFECTURE (JP)
TANAKA YOSHIKAZU (JP)
NAKAMURA NORIKO (JP)
KOBAYASHI HITOSHI (JP)
OKUHARA HIROAKI (JP)
KONDO MASAYOSHI (JP)
KOIKE YOSUKE (JP)
HOSHI YOSUKE (JP)
NOMIZU TOSHIKAZU (JP)
NIIGATA PREFECTURE (JP)
TANAKA YOSHIKAZU (JP)
NAKAMURA NORIKO (JP)
KOBAYASHI HITOSHI (JP)
OKUHARA HIROAKI (JP)
KONDO MASAYOSHI (JP)
KOIKE YOSUKE (JP)
HOSHI YOSUKE (JP)
NOMIZU TOSHIKAZU (JP)
International Classes:
A01H1/00; A01H5/00; C12N15/09
Domestic Patent References:
| WO2009062253A1 | 2009-05-22 | |||
| WO1994028140A1 | 1994-12-08 | |||
| WO2005017147A1 | 2005-02-24 | |||
| WO2004020637A1 | 2004-03-11 | |||
| WO2009062253A1 | 2009-05-22 | |||
| WO1996036716A1 | 1996-11-21 | |||
| WO2007094521A1 | 2007-08-23 | |||
| WO2008156214A1 | 2008-12-24 | |||
| WO2001092509A1 | 2001-12-06 |
Foreign References:
| JPH08511683A | 1996-12-10 | |||
| AU1100265W | ||||
| JPH08511683A | 1996-12-10 | |||
| JP2004001958W | 2004-02-20 | |||
| JP2008253250A | 2008-10-23 | |||
| AU1101111W | ||||
| AU2011001694W | ||||
| AU1100296W | ||||
| JP2007053342W | 2007-02-16 | |||
| JP2008061603W | 2008-06-19 | |||
| JP2008276029A | 2008-11-13 |
Other References:
TANAKA Y. ET AL.: "Flower color modification by engineering of the flavonoid biosynthetic pathway: Pratical perspectives", BIOSCI. BIOTECHNOL.BIOCHEM., vol. 74, no. 9, 2010, pages 1760 - 1769, XP002718419
MARTENS S. ET AL.: "Impact of biochemical pre-studies on specific metabolic engineering strategies of flavonoid biosynthesis in plant tissues", BIOCHEM.ENGINEER.J., vol. 14, 2003, pages 227 - 235, XP055095748
SHIRO MORI ET AL.: "Agrobacterium-ho ni yoru Hototogisu (Tricyrilis hirta) eno Anthocyanin Seigosei Keiro Kanren Idenshi no Donyu", JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, vol. 74, 1, 2005, pages 502
OKINAKA Y. ET AL.: "Selective accumulation of delphinidin derivatives in Tabacco using a putative flavonoid 3',5'-hydroxylase cDNA from campanula medium", BIOSCI.BIOTECHNOL.BIOCHEM., vol. 67, no. 1, 2003, pages 161 - 165, XP055095775
See also references of EP 2617283A4
PLANT J., vol. 54, 2008, pages 737 - 749
NATURE, vol. 366, 1993, pages 276 - 279
FEBS LETT., vol. 461, 1999, pages 241 - 245
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 67, no. 1, 2003, pages 161 - 165
PLANT BIOTECHNOLOGY, vol. 23, 2006, pages 5 - 11
PROTEIN, NUCLEIC ACID AND ENZYME, vol. 34, 1989, pages 1873 - 1878
TRENDS IN GENETICS, vol. 4, 1988, pages 13 - 18
"Annual Report No. 1-26 of the Niigata Agricultural Research Institute", PROJECT FOR THE DEVELOPMENT OF TOP BRAND AGRICULTURAL PRODUCTS, CULTIVATION OF BLUE LILIES, 2006
PLANT CELL REPORTS, vol. 22, 2004, pages 415 - 421
PLANT CELL REPORTS, vol. 22, 2004, pages 359 - 364
THEOR. APP. GENET., vol. 99, 1999, pages 383 - 390
PLANT CELL PHYSIOL., vol. 37, 1996, pages 49 - 59
CLONTECH, MOLECULAR BREEDING, vol. 11, 2003, pages 287 - 293
J. JAPAN SOC. HORT. SCI., vol. 77, 2008, pages 94 - 102
PLANT J., vol. 54, 2008, pages 949 - 962
"Abstracts of the 2010 Annual Meeting of the Japanese Society of Plant Physiologists", POSTER NO. P1C012(600, Retrieved from the Internet
HORT. SCIENCE, vol. 36, 2001, pages 384
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
PLANT BIOTECHNOLOGY, vol. 27, 2010, pages 375 - 383
MARTENS S. ET AL.: "Impact of biochemical pre-studies on specific metabolic engineering strategies of flavonoid biosynthesis in plant tissues", BIOCHEM.ENGINEER.J., vol. 14, 2003, pages 227 - 235, XP055095748
SHIRO MORI ET AL.: "Agrobacterium-ho ni yoru Hototogisu (Tricyrilis hirta) eno Anthocyanin Seigosei Keiro Kanren Idenshi no Donyu", JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, vol. 74, 1, 2005, pages 502
OKINAKA Y. ET AL.: "Selective accumulation of delphinidin derivatives in Tabacco using a putative flavonoid 3',5'-hydroxylase cDNA from campanula medium", BIOSCI.BIOTECHNOL.BIOCHEM., vol. 67, no. 1, 2003, pages 161 - 165, XP055095775
See also references of EP 2617283A4
PLANT J., vol. 54, 2008, pages 737 - 749
NATURE, vol. 366, 1993, pages 276 - 279
FEBS LETT., vol. 461, 1999, pages 241 - 245
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 67, no. 1, 2003, pages 161 - 165
PLANT BIOTECHNOLOGY, vol. 23, 2006, pages 5 - 11
PROTEIN, NUCLEIC ACID AND ENZYME, vol. 34, 1989, pages 1873 - 1878
TRENDS IN GENETICS, vol. 4, 1988, pages 13 - 18
"Annual Report No. 1-26 of the Niigata Agricultural Research Institute", PROJECT FOR THE DEVELOPMENT OF TOP BRAND AGRICULTURAL PRODUCTS, CULTIVATION OF BLUE LILIES, 2006
PLANT CELL REPORTS, vol. 22, 2004, pages 415 - 421
PLANT CELL REPORTS, vol. 22, 2004, pages 359 - 364
THEOR. APP. GENET., vol. 99, 1999, pages 383 - 390
PLANT CELL PHYSIOL., vol. 37, 1996, pages 49 - 59
CLONTECH, MOLECULAR BREEDING, vol. 11, 2003, pages 287 - 293
J. JAPAN SOC. HORT. SCI., vol. 77, 2008, pages 94 - 102
PLANT J., vol. 54, 2008, pages 949 - 962
"Abstracts of the 2010 Annual Meeting of the Japanese Society of Plant Physiologists", POSTER NO. P1C012(600, Retrieved from the Internet
HORT. SCIENCE, vol. 36, 2001, pages 384
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
PLANT BIOTECHNOLOGY, vol. 27, 2010, pages 375 - 383
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
Download PDF:
Claims: