Title:
METHOD FOR CULTURING LACTIC ACID BACTERIUM AND METHOD FOR PRODUCING FERMENTED MILK
Document Type and Number:
WIPO Patent Application WO/2010/113815
Kind Code:
A1
Abstract:
A concentrated cell suspension which is prepared by: adding a protease to an aqueous whey solution to thereby degrade protein in the aqueous whey solution; adding yeast extract or the like to the aqueous whey solution to give a whey degradation medium; inoculating the whey degradation medium with a lactic acid bacterium capable of producing a bacteriocin; culturing the lactic acid bacterium while maintaining the whey degradation medium at pH 5-6; and, after the completion of the culture, centrifuging the whey degradation medium (liquid culture medium) to separate a concentrated cell suspension containing the lactic acid bacterium cells in a concentrated form. This concentrated cell suspension, which shows an extremely high antibacterial activity (several tens of thousands AU), is usable as a food preservative. A yogurt which is produced by fermenting a yogurt mix which contains 0.01 to 0.1 wt% of the concentrated cell suspension. Thus, an increase in the acidity of the yogurt can be prevented.
Inventors:
ISAWA KAKUHEI (JP)
KAMIJO MASAYUKI (JP)
KAMIJO MASAYUKI (JP)
Application Number:
PCT/JP2010/055438
Publication Date:
October 07, 2010
Filing Date:
March 26, 2010
Export Citation:
Assignee:
MEIJI DAIRIES CORP (JP)
ISAWA KAKUHEI (JP)
KAMIJO MASAYUKI (JP)
ISAWA KAKUHEI (JP)
KAMIJO MASAYUKI (JP)
International Classes:
C12N1/20; A23C9/123
Domestic Patent References:
| WO2007138993A1 | 2007-12-06 | |||
| WO2010001580A1 | 2010-01-07 |
Foreign References:
| JP2008005834A | 2008-01-17 | |||
| JPH1057031A | 1998-03-03 | |||
| JPH08187071A | 1996-07-23 | |||
| JP2004283109A | 2004-10-14 | |||
| JPH04211360A | 1992-08-03 | |||
| JPH08187071A | 1996-07-23 | |||
| JP2004283109A | 2004-10-14 |
Other References:
See also references of EP 2415858A4
Attorney, Agent or Firm:
UEBA Hidetoshi et al. (JP)
Hidetoshi Ueha (JP)
Hidetoshi Ueha (JP)
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