Title:
METHOD FOR DEMONSTRATION OF A MOLECULAR EVENT IN A CELL BY MEANS OF FLUORESCENT MARKER PROTEINS
Document Type and Number:
WIPO Patent Application WO2005012913
Kind Code:
A3
Abstract:
The invention relates to a method for demonstration of the occurrence of a molecular event, particularly in a cell, characterised by the detection of the "solubilisation" of a fixed protein marker (or the fixing of a solubilised protein marker) which is a direct or indirect marker for the occurrence of the particular molecular event. Said protein marker is present in the cell before the above detection, the cell being subjected to a permeabilisation of the plasma membrane before detection, which liberates the solubilised protein into the extracellular medium, the presence of the marker protein thus being detected in the cell or the extra-cellular medium by any appropriate means, which permits the detection of whether the solubilisation, or the fixing have taken place and, hence, the corresponding molecular event.
Inventors:
ICHAS FRANCOIS (FR)
DE GIORGI ICHAS FRANCESCA (FR)
PIAZZA PIER-VINCENZO (FR)
DESSOLIN JEAN (FR)
SCHEMBRI LAURA (FR)
TOMASELLO FLORA (FR)
LARTIGUE LYDIA (FR)
DE GIORGI ICHAS FRANCESCA (FR)
PIAZZA PIER-VINCENZO (FR)
DESSOLIN JEAN (FR)
SCHEMBRI LAURA (FR)
TOMASELLO FLORA (FR)
LARTIGUE LYDIA (FR)
Application Number:
PCT/FR2004/001678
Publication Date:
April 21, 2005
Filing Date:
June 30, 2004
Export Citation:
Assignee:
INST NAT SANTE RECH MED (FR)
ICHAS FRANCOIS (FR)
DE GIORGI ICHAS FRANCESCA (FR)
PIAZZA PIER-VINCENZO (FR)
DESSOLIN JEAN (FR)
SCHEMBRI LAURA (FR)
TOMASELLO FLORA (FR)
LARTIGUE LYDIA (FR)
ICHAS FRANCOIS (FR)
DE GIORGI ICHAS FRANCESCA (FR)
PIAZZA PIER-VINCENZO (FR)
DESSOLIN JEAN (FR)
SCHEMBRI LAURA (FR)
TOMASELLO FLORA (FR)
LARTIGUE LYDIA (FR)
International Classes:
G01N33/50; G01N33/573; G01N33/68; (IPC1-7): G01N33/573; G01N33/68; G01N33/50; G01N33/542; C12Q1/37
Domestic Patent References:
| WO2002094778A2 | 2002-11-28 | |||
| WO2000073437A1 | 2000-12-07 |
Foreign References:
| US20030100027A1 | 2003-05-29 | |||
| US20020150885A1 | 2002-10-17 |
Other References:
ABDULKARIM BASSAM ET AL: "Antiviral agent Cidofovir decreases Epstein-Barr virus (EBV) oncoproteins and enhances the radiosensitivity in EBV-related malignancies.", ONCOGENE, vol. 22, no. 15, 17 April 2003 (2003-04-17), pages 2260 - 2271, XP002311595, ISSN: 0950-9232
WOLTER KEITH G ET AL: "Movement of Bax from the cytosol to mitochondria during apoptosis", JOURNAL OF CELL BIOLOGY, vol. 139, no. 5, 1 December 1997 (1997-12-01), pages 1281 - 1292, XP002311593, ISSN: 0021-9525
AYUKAWA KOICHI ET AL: "La autoantigen is cleaved in the COOH terminus and loses the nuclear localization signal during apoptosis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 44, 3 November 2000 (2000-11-03), pages 34465 - 34470, XP002311594, ISSN: 0021-9258
DEAK JOSEPH C ET AL: "Fas-induced proteolytic activation and intracellular redistribution of the stress-signaling kinase MEKK1", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, no. 10, 12 May 1998 (1998-05-12), pages 5595 - 5600, XP002311596, ISSN: 0027-8424
ZHANG X ET AL: "APOPTOSIS INDUCTION IN PROSTATE CANCER CELLS BY A NOVEL GENE PRODUCT, PHYDE, INVOLVES CASPASE-3", ONCOGENE, BASINGSTOKE, HANTS, GB, vol. 20, no. 42, 20 September 2001 (2001-09-20), pages 5982 - 5990, XP008003138, ISSN: 0950-9232
DE GIORGI FRANCESAA ET AL: "The permeability transition pore signals apoptosis by directing Bax translocation and multimerization.", THE FASEB JOURNAL : OFFICIAL PUBLICATION OF THE FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY. APR 2002, vol. 16, no. 6, April 2002 (2002-04-01), pages 607 - 609, XP002311597, ISSN: 1530-6860
GU J ET AL: "A novel single tetracycline-regulative adenoviral vector for tumor-specific Bax gene expression and cell killing in vitro and in vivo", ONCOGENE, BASINGSTOKE, HANTS, GB, vol. 21, no. 31, 2002, pages 4757 - 4764, XP002979568, ISSN: 0950-9232
WOLTER KEITH G ET AL: "Movement of Bax from the cytosol to mitochondria during apoptosis", JOURNAL OF CELL BIOLOGY, vol. 139, no. 5, 1 December 1997 (1997-12-01), pages 1281 - 1292, XP002311593, ISSN: 0021-9525
AYUKAWA KOICHI ET AL: "La autoantigen is cleaved in the COOH terminus and loses the nuclear localization signal during apoptosis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 44, 3 November 2000 (2000-11-03), pages 34465 - 34470, XP002311594, ISSN: 0021-9258
DEAK JOSEPH C ET AL: "Fas-induced proteolytic activation and intracellular redistribution of the stress-signaling kinase MEKK1", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, no. 10, 12 May 1998 (1998-05-12), pages 5595 - 5600, XP002311596, ISSN: 0027-8424
ZHANG X ET AL: "APOPTOSIS INDUCTION IN PROSTATE CANCER CELLS BY A NOVEL GENE PRODUCT, PHYDE, INVOLVES CASPASE-3", ONCOGENE, BASINGSTOKE, HANTS, GB, vol. 20, no. 42, 20 September 2001 (2001-09-20), pages 5982 - 5990, XP008003138, ISSN: 0950-9232
DE GIORGI FRANCESAA ET AL: "The permeability transition pore signals apoptosis by directing Bax translocation and multimerization.", THE FASEB JOURNAL : OFFICIAL PUBLICATION OF THE FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY. APR 2002, vol. 16, no. 6, April 2002 (2002-04-01), pages 607 - 609, XP002311597, ISSN: 1530-6860
GU J ET AL: "A novel single tetracycline-regulative adenoviral vector for tumor-specific Bax gene expression and cell killing in vitro and in vivo", ONCOGENE, BASINGSTOKE, HANTS, GB, vol. 21, no. 31, 2002, pages 4757 - 4764, XP002979568, ISSN: 0950-9232
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