Title:
METHOD FOR DESALTING POLYPEPTIDES
Document Type and Number:
WIPO Patent Application WO/2021/129016
Kind Code:
A1
Abstract:
Disclosed is a method for desalting polypeptides, the method comprising loading a polypeptide sample onto a preparative column, rinsing the column with mobile phases A1 and B, then rinsing the column with mobile phases A2 and B, and finally performing gradient elution with A2 and B; collecting the sample; performing rotary evaporation under reduced pressure; and freeze-drying the product to obtain desalted refined polypeptides. In the present method, salt transfer can be performed on a reversed-phase high-efficiency preparative chromatography system by taking a silica filler with a bonded alkyl group as the stationary phase. The method solves the problem of it being difficult to elute samples during desalting without needing additional equipment and materials, and during salt transfer, can further remove impurities, improve sample purity, reduce sample volume after salt transfer, and increase sample concentration. The whole process is simple to operate and has a short production cycle, which is conducive to large-scale industrial production.
Inventors:
JIANG XUBANG (CN)
HUANG JIACHENG (CN)
YIN CHUANLONG (CN)
TAO ANJIN (CN)
YU PINXIANG (CN)
HUANG JIACHENG (CN)
YIN CHUANLONG (CN)
TAO ANJIN (CN)
YU PINXIANG (CN)
Application Number:
PCT/CN2020/118280
Publication Date:
July 01, 2021
Filing Date:
September 28, 2020
Export Citation:
Assignee:
HYBIO PHARMACEUTICAL CO LTD (CN)
International Classes:
C07K1/16
Domestic Patent References:
| WO2017162653A1 | 2017-09-28 | |||
| WO2019227342A1 | 2019-12-05 |
Foreign References:
| CN109810177A | 2019-05-28 | |||
| CN110157761A | 2019-08-23 | |||
| CN107525880A | 2017-12-29 | |||
| CN108640985A | 2018-10-12 | |||
| CN110028573A | 2019-07-19 | |||
| CN105777872A | 2016-07-20 |
Attorney, Agent or Firm:
SHENPAT INTELLECTUAL PROPERTY AGENCY (CN)
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