Title:
METHOD FOR DETECTING ANALYTE USING POLYNUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO/2023/243694
Kind Code:
A1
Abstract:
The present invention pertains to a method for detecting an analyte contained in a sample, the method comprising: (1) mixing the sample and salt with a gold-nanoparticle-labeled aptamer in which gold nanoparticles are bound to an aptamer capable of binding to the analyte; and (2) judging that the sample contains the analyate when there is no aggregation of gold-nanoparticle-labeled aptamer in the mixture obtained by (1).
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Inventors:
IKEBUKURO KAZUNORI (JP)
SAITO HIROSHI (JP)
OHNISHI NORIYUKI (JP)
SAITO HIROSHI (JP)
OHNISHI NORIYUKI (JP)
Application Number:
PCT/JP2023/022324
Publication Date:
December 21, 2023
Filing Date:
June 15, 2023
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION TOKYO UNIV OF AGRICULTURE AND TECHNOLOGY (JP)
JNC CORP (JP)
JNC CORP (JP)
International Classes:
C12N15/115; C12Q1/68; G01N33/543; G01N33/553; G01N33/569
Domestic Patent References:
| WO2022016010A2 | 2022-01-20 |
Other References:
JORGE L. CHáVEZ; ROBERT I. MACCUSPIE; MORLEY O. STONE; NANCY KELLEY-LOUGHNANE: "Colorimetric detection with aptamer–gold nanoparticle conjugates: effect of aptamer length on response", JOURNAL OF NANOPARTICLE RESEARCH ; AN INTERDISCIPLINARY FORUM FOR NANOSCALE SCIENCE AND TECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 14, no. 10, 14 September 2012 (2012-09-14), Do , pages 1 - 11, XP035125718, ISSN: 1572-896X, DOI: 10.1007/s11051-012-1166-0
ABRAHAM KEVIN M., ROUEINFAR MINA, PONCE ALEX T., LUSSIER MIA E., BENSON DANICA B., HONG KA LOK: "In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine", ACS OMEGA, ACS PUBLICATIONS, US, vol. 3, no. 10, 31 October 2018 (2018-10-31), US , pages 13576 - 13583, XP093117601, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01859
WEIAN ZHAO, WILLIAM CHIUMAN, JEFFREY C. F. LAM, SIMON A. MCMANUS, WEI CHEN, YUGUO CUI, ROBERT PELTON, MICHAEL A. BROOK, YINGFU LI: "DNA Aptamer Folding on Gold Nanoparticles: From Colloid Chemistry to Biosensors", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 130, no. 11, 1 March 2008 (2008-03-01), pages 3610 - 3618, XP055236780, ISSN: 0002-7863, DOI: 10.1021/ja710241b
ABRAHAM KEVIN M., ROUEINFAR MINA, PONCE ALEX T., LUSSIER MIA E., BENSON DANICA B., HONG KA LOK: "In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine", ACS OMEGA, ACS PUBLICATIONS, US, vol. 3, no. 10, 31 October 2018 (2018-10-31), US , pages 13576 - 13583, XP093117601, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01859
WEIAN ZHAO, WILLIAM CHIUMAN, JEFFREY C. F. LAM, SIMON A. MCMANUS, WEI CHEN, YUGUO CUI, ROBERT PELTON, MICHAEL A. BROOK, YINGFU LI: "DNA Aptamer Folding on Gold Nanoparticles: From Colloid Chemistry to Biosensors", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 130, no. 11, 1 March 2008 (2008-03-01), pages 3610 - 3618, XP055236780, ISSN: 0002-7863, DOI: 10.1021/ja710241b
Attorney, Agent or Firm:
EIKOH, P.C. (JP)
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