Title:
METHOD FOR DETECTING ANTIGEN ON MEMBRANE BY USING FLUORESCENCE RESONANCE ENERGY TRANSFER IMMUNOASSAY
Document Type and Number:
WIPO Patent Application WO/2017/200308
Kind Code:
A1
Abstract:
A method for detecting an antigen according to an embodiment of the present invention comprises the steps of: (a) contacting an antibody against an antigen to be detected with a specimen containing an antigen to be detected on a diagnostic device to react the antibody and the sample, the diagnostic device being formed of a membrane containing the antibody at one end thereof and the specimen at another end thereof; (b) irradiating light on the antigen-antibody conjugate formed in step (a) to give rise to fluorescence resonance energy transfer to obtain a resulting spectrum; and (c) analyzing the spectrum obtained in step (b) to determine the presence or absence of the antigen to be detected and to measure a concentration of the antigen.
Inventors:
KIM MIN GON (KR)
OH HYUNKYUNG (KR)
OH HYUNKYUNG (KR)
Application Number:
PCT/KR2017/005148
Publication Date:
November 23, 2017
Filing Date:
May 18, 2017
Export Citation:
Assignee:
GWANGJU INST SCIENCE & TECH (KR)
International Classes:
G01N33/53; G01N21/64; G01N33/558; G01N33/68
Foreign References:
| KR20140058305A | 2014-05-14 | |||
| JP2007064766A | 2007-03-15 | |||
| JP2013120120A | 2013-06-17 | |||
| KR20130037648A | 2013-04-16 |
Other References:
LI, TAIHUA ET AL.: "Label-free Homogeneous FRET Immunoassay for the Detection of Mycotoxins that Utilizes Quenching of the intrinsic Fluorescence of Antibodies", BIOSENSORS AND BIOELECTRONICS, vol. 42, 2 November 2012 (2012-11-02), pages 403 - 408, XP055439321
Attorney, Agent or Firm:
SHIN, Yong Hyun et al. (KR)
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