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Title:
METHOD FOR DETECTING BACTERIA IN BLOOD SAMPLE
Document Type and Number:
WIPO Patent Application WO/2019/188924
Kind Code:
A1
Abstract:
The problem of the present invention is to provide a highly convenient and prompt method for detecting existence of bacteria in blood samples with high sensitivity. By using a method comprising sequential processes (a) to (d), wherein the process (a) mixes a culture of a subject-originated blood sample with ammonium chloride and obtains a hemolysed sample; the process (b) filters the hemolysed sample with a filter membrane having pore diameter in which bacteria cannot be filtered; the process (c) collects residue caught on the filter membrane after filtering and obtains a residue sample; and the process (d) analyzes existence of bacteria in the residue sample by using a mass spectrometer; the matter of bacterial infection of the subject can be detected with high sensitivity.

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Inventors:
MURATA SYOTA (JP)
TSUCHIDA SACHIO (JP)
MIYABE AKIKO (JP)
SATOH MAMORU (JP)
MATSUSHITA KAZUYUKI (JP)
NOMURA FUMIO (JP)
Application Number:
PCT/JP2019/012397
Publication Date:
October 03, 2019
Filing Date:
March 25, 2019
Export Citation:
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Assignee:
UNIV CHIBA NAT UNIV CORP (JP)
International Classes:
C12Q1/04; G01N27/62
Domestic Patent References:
WO2011058131A12011-05-19
Foreign References:
JP2012532618A2012-12-20
JP2012507711A2012-03-29
US20160251694A12016-09-01
Other References:
PROD'HOM, G. ET AL.: "Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 48, no. 4, 2010, pages 1481 - 1483, XP002626591, doi:10.1128/JCM.01780-09
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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