Title:
METHOD FOR DETECTING BIOMATERIAL USING MAGNETIC MICROPARTICLES AND SURFACE-ENHANCED RAMAN SIGNALS
Document Type and Number:
WIPO Patent Application WO/2022/108218
Kind Code:
A1
Abstract:
The present invention relates to a method for the simultaneous detection of multiple biomaterials using Raman signals and magnetic microparticles (MMP) modified with a target capture probe. More specifically, the method comprises a step in which the modified MMP is hybridized with a biomaterial-specific target sequence to be detected and a signal probe, fluorescence signals are detected from each of a solution containing the hybridized MMP and a signal probe emitted from the MMP solution, and then metal nanoparticles are added to the emitted signal probe to detect Raman signals, and thus, the method can specifically detect two or more types of biomaterials with high sensitivity. The present invention is characterized in that fluorescence signals and Raman signals can be obtained with high sensitivity, a step for separating and then washing MMPs is simple, thus enabling multiple detection using various probes, and a hybridization step and a signal detection step are separated, thus reducing non-specific binding and increasing biomaterial detection efficiency.
Inventors:
LIM DONG-KWON (KR)
Application Number:
PCT/KR2021/016288
Publication Date:
May 27, 2022
Filing Date:
November 10, 2021
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12Q1/6816; C12Q1/6883
Foreign References:
| KR101712429B1 | 2017-03-08 | |||
| KR20160119318A | 2016-10-13 | |||
| KR20160086768A | 2016-07-20 | |||
| KR20150008291A | 2015-01-22 |
Other References:
KIM, BONGSU: "Development of Multi-Pathogen Diagnostic Sensor using Gold Nanowires", THE SCIENCE & TECHNOLOGY, 1 May 2010 (2010-05-01), pages 56 - 58, XP009536802, ISSN: 1599-7340
HWANG MIN JI; JANG AH SEONG; LIM DONG-KWON: "Comparative study of fluorescence and surface-enhanced Raman scattering with magnetic microparticle-based assay for target bacterial DNA detection", SENSORS AND ACTUATORS B: CHEMICAL, ELSEVIER BV, NL, vol. 329, 1 November 2020 (2020-11-01), NL , XP086455467, ISSN: 0925-4005, DOI: 10.1016/j.snb.2020.129134
HWANG MIN JI; JANG AH SEONG; LIM DONG-KWON: "Comparative study of fluorescence and surface-enhanced Raman scattering with magnetic microparticle-based assay for target bacterial DNA detection", SENSORS AND ACTUATORS B: CHEMICAL, ELSEVIER BV, NL, vol. 329, 1 November 2020 (2020-11-01), NL , XP086455467, ISSN: 0925-4005, DOI: 10.1016/j.snb.2020.129134
Attorney, Agent or Firm:
PADO IP LAW PLLC (KR)
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