Title:
METHOD FOR DETECTING FLUORESCENCE OR ABSORBANCE, METHOD FOR SUPPRESSING BACKGROUND, METHOD FOR MEASURING ADP, METHOD FOR MEASURING ACTIVITY OF ADP-SYNTHESIZING ENZYME, AND METHOD FOR MEASURING ACTIVITY OF GLUCOSYLTRANSFERASE
Document Type and Number:
WIPO Patent Application WO/2015/076361
Kind Code:
A1
Abstract:
A method for detecting fluorescence or absorbance according to the present invention is characterized by comprising reducing resazurin into resorufin by a diaphorase in the presence of an SH reagent and NADH or NADPH and then measuring the resultant fluorescence intensity or absorbance. A method for measuring ADP according to the present invention is characterized by comprising: step (2-1) for treating glucose with ADP and ADP-dependent hexokinase; step (2-2) for treating glucose-6-phosphate obtained in the above step (2-1) with NAD or NADP and glucose-6-phosphate dehydrogenase; and step (2-3) for treating resazurin with NADH or NADPH obtained in the above step (2-2) and a diaphorase in the presence of an SH reagent and then measuring the resultant fluorescence intensity or absorbance.
Inventors:
KUMAGAI KAZUO (JP)
OKABE TAKAYOSHI (JP)
KOJIMA HIROTATSU (JP)
NAGANO TETSUO (JP)
OKABE TAKAYOSHI (JP)
KOJIMA HIROTATSU (JP)
NAGANO TETSUO (JP)
Application Number:
PCT/JP2014/080865
Publication Date:
May 28, 2015
Filing Date:
November 21, 2014
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12Q1/26; C12Q1/32; C12Q1/48; G01N21/78
Foreign References:
| JP2005021025A | 2005-01-27 | |||
| JPH05207895A | 1993-08-20 | |||
| JPS62239999A | 1987-10-20 | |||
| JP2005500406A | 2005-01-06 | |||
| JP2005501228A | 2005-01-13 | |||
| JP2005046029A | 2005-02-24 | |||
| JPH09234098A | 1997-09-09 | |||
| JP2011103825A | 2011-06-02 | |||
| US7338775B1 | 2008-03-04 |
Other References:
TAKUJI SHODA ET AL.: "Kakushu Maleimide-ki o Yusuru Keiko Shikiso no Hannosei to Kogaku Tokusei", ABSTRACTS OF ANNUAL MEETING OF PHARMACEUTICAL SOCIETY OF JAPAN, vol. 126, no. 2, 2006, pages 42, XP008184635
TAKUYA MATSUMOTO ET AL.: "A Novel Fluorescence Probe for Thiol Modification", ABSTRACTS OF ANNUAL MEETING OF PHARMACEUTICAL SOCIETY OF JAPAN, vol. 128, no. 4, 2008, pages 7, XP008184634
See also references of EP 3072975A4
"Dictionary of Biochemistry, 4th Edition,", 2007, TOKYO KAGAKU DOJIN, pages: 931
"Essentials of Glycobiology, 2nd edition,", 2009, COLD SPRING HARBOR LABORATORY PRESS, article "Chapter 4,"
"Sugar chain engineering can be seen properly", 2008, HAKUJITSUSHA
CHEMBIOCHEM, vol. 11, no. 14, 2010, pages 1939 - 1949
ANAL. BIOCHEM, vol. 102, no. 2, 1980, pages 441 - 449
ANAL. BIOCHEM, vol. 220, no. 1, 1994, pages 92 - 97
GLYCOBIOLOGY, vol. 21, no. 6, 2011, pages 727 - 733
ANGEW. CHEM. INT. ED., vol. 50, no. 52, 2011, pages 12534 - 12537
"Dictionary of Biochemistry, 4th Edition,", 2007, TOKYO KAGAKU DOJIN, pages: 340
"Experimental Medicine", vol. 27, 2009, YODOSHA, article "New challenge for molecularly targeted drug development, drug discovery story of potential molecularly targeted drug and new drug development trend to next-generation drug discovery technology"
ARCH. BIOCHEM. BIOPHYS, vol. 425, no. 1, 2004, pages 51 - 57
ARCH. BIOCHEM. BIOPHYS, vol. 425, no. L, 2004, pages 51 - 57
J. BIOL. CHEM, vol. 265, no. 11, 1990, pages 6339 - 6345
TAKUYA MATSUMOTO ET AL.: "A Novel Fluorescence Probe for Thiol Modification", ABSTRACTS OF ANNUAL MEETING OF PHARMACEUTICAL SOCIETY OF JAPAN, vol. 128, no. 4, 2008, pages 7, XP008184634
See also references of EP 3072975A4
"Dictionary of Biochemistry, 4th Edition,", 2007, TOKYO KAGAKU DOJIN, pages: 931
"Essentials of Glycobiology, 2nd edition,", 2009, COLD SPRING HARBOR LABORATORY PRESS, article "Chapter 4,"
"Sugar chain engineering can be seen properly", 2008, HAKUJITSUSHA
CHEMBIOCHEM, vol. 11, no. 14, 2010, pages 1939 - 1949
ANAL. BIOCHEM, vol. 102, no. 2, 1980, pages 441 - 449
ANAL. BIOCHEM, vol. 220, no. 1, 1994, pages 92 - 97
GLYCOBIOLOGY, vol. 21, no. 6, 2011, pages 727 - 733
ANGEW. CHEM. INT. ED., vol. 50, no. 52, 2011, pages 12534 - 12537
"Dictionary of Biochemistry, 4th Edition,", 2007, TOKYO KAGAKU DOJIN, pages: 340
"Experimental Medicine", vol. 27, 2009, YODOSHA, article "New challenge for molecularly targeted drug development, drug discovery story of potential molecularly targeted drug and new drug development trend to next-generation drug discovery technology"
ARCH. BIOCHEM. BIOPHYS, vol. 425, no. 1, 2004, pages 51 - 57
ARCH. BIOCHEM. BIOPHYS, vol. 425, no. L, 2004, pages 51 - 57
J. BIOL. CHEM, vol. 265, no. 11, 1990, pages 6339 - 6345
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
Masatake Shiga (JP)
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