Title:
METHOD FOR DETECTING G-QUADRUPLEX FORMATION
Document Type and Number:
WIPO Patent Application WO/2013/021536
Kind Code:
A1
Abstract:
The purpose of the method according to the present invention is to specifically detect G-quadruplex formation even in the presence of potassium ion.
According to the method of the present invention, the presence or absence of G-quadruplex formation by a target DNA is determined utilizing a phenomenon that thioflavin T emits strong fluorescence when reacted with G-quadruplex in the presence of potassium ion. The method of the present invention comprises: maintaining a first sample solution, which contains potassium ion and the target DNA, under such conditions as allowing G-quadruplex formation; adding thioflavin T thereto; measuring a first fluorescence intensity; maintaining a second sample solution containing the target DNA under such conditions as making G-quadruplex formation unstable; adding thioflavin T thereto; measuring a second fluorescence intensity; and, in the case where the difference between these intensities is 0 or greater, determining the target DNA as being capable of forming G-quadruplex.
Inventors:
MIYOSHI DAISUKE
Application Number:
PCT/JP2012/003921
Publication Date:
February 14, 2013
Filing Date:
June 15, 2012
Export Citation:
Assignee:
PANASONIC CORP (JP)
MIYOSHI DAISUKE
MIYOSHI DAISUKE
International Classes:
C12Q1/68; C12N15/09; G01N21/78
Domestic Patent References:
| WO2009144914A1 | 2009-12-03 |
Foreign References:
| JP2010030999A | 2010-02-12 |
Other References:
LUBITZ I. ET AL.: "Specific high-affinity binding of thiazole orange to triplex and G- quadruplex DNA", BIOCHEMISTRY, vol. 49, no. 17, 2010, pages 3567 - 3574
KOVALSKA VB. ET AL.: "Mono and trimethine cyanines Cyan 40 and Cyan 2 as probes for highly selective fluorescent detection of non-canonical DNA structures", J. FLUORESC., vol. 21, no. 1, January 2011 (2011-01-01), pages 223 - 230
CHANG CC. ET AL.: "Detection of quadruplex DNA structures in human telomeres by a fluorescent carbazole derivative", ANAL. CHEM., vol. 76, no. 15, 2004, pages 4490 - 4494
CHANG TC. ET AL.: "Detection of G-quadruplexes in cells and investigation of G-quadruplex structure of d(T2AG3)4 in K+ solution by a carbazole derivative: BMVC", METHODS MOL. BIOL., vol. 608, 2010, pages 183 - 206
CANETE M. ET AL.: "A study of interaction of thioflavine T with DNA: evidence for intercalation", CELL. MOL. BIOL., vol. 33, no. 2, 1987, pages 191 - 199
KOVALSKA VB. ET AL.: "Mono and trimethine cyanines Cyan 40 and Cyan 2 as probes for highly selective fluorescent detection of non-canonical DNA structures", J. FLUORESC., vol. 21, no. 1, January 2011 (2011-01-01), pages 223 - 230
CHANG CC. ET AL.: "Detection of quadruplex DNA structures in human telomeres by a fluorescent carbazole derivative", ANAL. CHEM., vol. 76, no. 15, 2004, pages 4490 - 4494
CHANG TC. ET AL.: "Detection of G-quadruplexes in cells and investigation of G-quadruplex structure of d(T2AG3)4 in K+ solution by a carbazole derivative: BMVC", METHODS MOL. BIOL., vol. 608, 2010, pages 183 - 206
CANETE M. ET AL.: "A study of interaction of thioflavine T with DNA: evidence for intercalation", CELL. MOL. BIOL., vol. 33, no. 2, 1987, pages 191 - 199
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
Patent business corporation Owner old patent firm (JP)
Patent business corporation Owner old patent firm (JP)
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