Title:
METHOD FOR DETECTING GLUTEN
Document Type and Number:
WIPO Patent Application WO2006004394
Kind Code:
A3
Abstract:
Provided is a mAb-based method for the detection of T cell stimulatory epitopes known to be involved in CD. The method has many advantages compared to the existing methods for the detection of gluten since it is the first method that can; (i) detect T cell stimulatory epitopes of gluten; (ii) detect the epitopes separately, (iii) detect T cell stimulatory epitopes present on gliadin and glutenin homologues present in other cereals also known to be involved in CD; and (iv) detect T cell stimulatory epitopes on both intact proteins and small protein fragments. The new method is a valuable tool in the screening of basic ingredients, semi manufactured ingredients and food products that are intended to be used in the gluten free diet of CD patients. Moreover the new method can also be used for the screening of cereals and different wheat varieties for the level of toxicity for CD patients. Thereby the method can help in the selection of cereals and wheat varieties with low toxicity which might form the basis for future breeding programs. In the future these cereals will be used for the production of safe food for CD patients.
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Inventors:
DRIJFHOUT JAN WOUTER (NL)
KONING FRITS (NL)
SPAENIJ-DEKKING ELISABETH HERM (NL)
KONING FRITS (NL)
SPAENIJ-DEKKING ELISABETH HERM (NL)
Application Number:
PCT/NL2005/000400
Publication Date:
March 02, 2006
Filing Date:
June 01, 2005
Export Citation:
Assignee:
ACADEMISCH ZIEKENHUIS LEIDEN (NL)
DRIJFHOUT JAN WOUTER (NL)
KONING FRITS (NL)
SPAENIJ-DEKKING ELISABETH HERM (NL)
DRIJFHOUT JAN WOUTER (NL)
KONING FRITS (NL)
SPAENIJ-DEKKING ELISABETH HERM (NL)
International Classes:
G01N33/68; A23L7/10; G01N33/564
Domestic Patent References:
| WO2001075451A1 | 2001-10-11 | |||
| WO2002083722A2 | 2002-10-24 | |||
| WO2003066079A2 | 2003-08-14 | |||
| WO1997009620A1 | 1997-03-13 | |||
| WO2001025793A2 | 2001-04-12 |
Foreign References:
| EP0905518A1 | 1999-03-31 |
Other References:
DATABASE CGN [online] Wageningen UR; 21 June 2004 (2004-06-21), XP002359265, retrieved from WWW.CGN.WAGENINGEN-UR.NL Database accession no. CGN 10684
DATABASE CGN [online] Wageningen-UR; 21 June 2004 (2004-06-21), "Sappo", XP002359266, retrieved from WWW.CGN.WAGENINGEN--UR.NL Database accession no. CGN 12393
DATABASE CGN [online] Wageningen UR; 21 June 2004 (2004-06-21), "Weissahr Rotkorn Binkel", XP002359267, retrieved from WWW.CGN.WAGENINGEN-UR.NL Database accession no. CGN 04210
SPAENIJ-DEKKING E H A ET AL: "A novel and sensitive method for the detection of T cell stimulatory epitopes of alpha/beta- and gamma-gliadin", GUT, vol. 53, no. 9, September 2004 (2004-09-01), pages 1267 - 1273, XP008040242
SPAENIJ-DEKKING L ET AL: "Natural variation in toxicity of wheat: potential for selection of nontoxic varieties for celiac disease patients.", GASTROENTEROLOGY., vol. 129, no. 3, September 2005 (2005-09-01), pages 797 - 806, XP002359246
HILL A S ET AL: "MONOCLONAL ANTIBODY BASED TWO-SITE ENZYME IMMUNOASSAYS FOR WHEAT GLUTEN PROTEINS 1. KINETIC CHARACTERISTICS AND COMPARISON WITH OTHER ELISA FORMATS", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 1, no. 3, 1989, pages 147 - 160, XP008040240
SKERRITT J H ET AL: "CELLULAR AND HUMORAL RESPONSES IN COELIAC DISEASE. 2. PROTEIN EXTRACTS FROM DIFFERENT CEREALS", CLINICA CHIMICA ACTA, vol. 204, no. 1/3, 1991, pages 109 - 122, XP000673555
VADER W ET AL: "THE GLUTEN RESPONSE IN CHILDREN WITH CELIAC DISEASE IS DIRECTED TOWARD MULTIPLE GLIADIN AND GLUTENIN PEPTIDES", GASTROENTEROLOGY, vol. 122, no. 7, June 2000 (2000-06-01), pages 1729 - 1737, XP009013823
VADER LW ET AL: "Characterization of cereal toxicity for celiac disease patients based on protein homology in grains.", GASTROENTEROLOGY, vol. 125, no. 4, October 2003 (2003-10-01), pages 1105 - 1113, XP002359247
MOLBERG O ET AL: "Intestinal T-cell responses to high-molecular-weight glutenins in celiac disease.", GASTROENTEROLOGY, vol. 125, no. 2, August 2003 (2003-08-01), pages 337 - 344, XP002359248
MOLBERG O ET AL: "Mapping of gluten T-cell epitopes in the bread wheat ancestors: implications for celiac disease.", GASTROENTEROLOGY, vol. 128, no. 2, February 2005 (2005-02-01), pages 393 - 401, XP002359249
DATABASE CGN [online] Wageningen-UR; 21 June 2004 (2004-06-21), "Sappo", XP002359266, retrieved from WWW.CGN.WAGENINGEN--UR.NL Database accession no. CGN 12393
DATABASE CGN [online] Wageningen UR; 21 June 2004 (2004-06-21), "Weissahr Rotkorn Binkel", XP002359267, retrieved from WWW.CGN.WAGENINGEN-UR.NL Database accession no. CGN 04210
SPAENIJ-DEKKING E H A ET AL: "A novel and sensitive method for the detection of T cell stimulatory epitopes of alpha/beta- and gamma-gliadin", GUT, vol. 53, no. 9, September 2004 (2004-09-01), pages 1267 - 1273, XP008040242
SPAENIJ-DEKKING L ET AL: "Natural variation in toxicity of wheat: potential for selection of nontoxic varieties for celiac disease patients.", GASTROENTEROLOGY., vol. 129, no. 3, September 2005 (2005-09-01), pages 797 - 806, XP002359246
HILL A S ET AL: "MONOCLONAL ANTIBODY BASED TWO-SITE ENZYME IMMUNOASSAYS FOR WHEAT GLUTEN PROTEINS 1. KINETIC CHARACTERISTICS AND COMPARISON WITH OTHER ELISA FORMATS", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 1, no. 3, 1989, pages 147 - 160, XP008040240
SKERRITT J H ET AL: "CELLULAR AND HUMORAL RESPONSES IN COELIAC DISEASE. 2. PROTEIN EXTRACTS FROM DIFFERENT CEREALS", CLINICA CHIMICA ACTA, vol. 204, no. 1/3, 1991, pages 109 - 122, XP000673555
VADER W ET AL: "THE GLUTEN RESPONSE IN CHILDREN WITH CELIAC DISEASE IS DIRECTED TOWARD MULTIPLE GLIADIN AND GLUTENIN PEPTIDES", GASTROENTEROLOGY, vol. 122, no. 7, June 2000 (2000-06-01), pages 1729 - 1737, XP009013823
VADER LW ET AL: "Characterization of cereal toxicity for celiac disease patients based on protein homology in grains.", GASTROENTEROLOGY, vol. 125, no. 4, October 2003 (2003-10-01), pages 1105 - 1113, XP002359247
MOLBERG O ET AL: "Intestinal T-cell responses to high-molecular-weight glutenins in celiac disease.", GASTROENTEROLOGY, vol. 125, no. 2, August 2003 (2003-08-01), pages 337 - 344, XP002359248
MOLBERG O ET AL: "Mapping of gluten T-cell epitopes in the bread wheat ancestors: implications for celiac disease.", GASTROENTEROLOGY, vol. 128, no. 2, February 2005 (2005-02-01), pages 393 - 401, XP002359249
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