Title:
METHOD OF DETECTING HISTAMINE-PRODUCING BACTERIUM AND PRIMER FOR USE IN THE METHOD
Document Type and Number:
WIPO Patent Application WO/2004/094632
Kind Code:
A1
Abstract:
A method of detecting a histamine-producing bacterium; a method of identifying the bacterial strain thereof; and a primer for use therein. In particular, PCR of a test sample is carried out with the use of a primer containing base sequences of SEQ ID NO. 1 and 2, and thus obtained PCR products are electrophoresed to thereby detect a histamine-producing bacterium. The strain of detected histamine-producing bacterium is identified by SSCP analysis. As a result, the histamine-producing bacterium can be detected accurately and easily by the detection method, and identification of a causative bacterium is facilitated by the identification method, thereby enabling speedy clarification of pollution source.
Inventors:
KIMURA BON (JP)
FUJII TAKEO (JP)
TAKAHASHI HAJIME (JP)
FUJII TAKEO (JP)
TAKAHASHI HAJIME (JP)
Application Number:
PCT/JP2004/005959
Publication Date:
November 04, 2004
Filing Date:
April 23, 2004
Export Citation:
Assignee:
KIMURA BON (JP)
FUJII TAKEO (JP)
TAKAHASHI HAJIME (JP)
FUJII TAKEO (JP)
TAKAHASHI HAJIME (JP)
International Classes:
G01N33/483; C12N15/09; C12Q1/04; C12Q1/68; G01N27/447; G01N33/50; (IPC1-7): C12N15/00; C12Q1/04; C12Q1/68; G01N27/26; G01N33/483; G01N33/50
Other References:
TAKAHASHI H. ET AL.: "Cloning and sequencing of the histidine decarboxylase genes of gram-negative, histamine-producing bacteria and their application in detection and identification of these oragnisms in fish", APPL. ENVIRON. MICROBIOL., vol. 69, no. 5, May 2003 (2003-05-01), pages 2568 - 2579, XP002984608
TAKAHASHI H.: "PCR-SSCP-ho ni yoru histamine seiseikin no kenshutsu.dotei system no kaihatsu", NIPPON SHOKUHIN EISEI GAKKAI GAKUJUTSU KOENKAI KOEN YOSHISHU, vol. 48, 2002, pages 97, XP002984609
FUJII T.: "Heisei 7,8 nendo histamine seiseikin no jinsoku kenshutsu.dotei system no kaihatsu", SHOKUHIN ANZENSEI KOJO NI KANSURU KENKYU SEIKA HOKOKUSHU, 1999, pages 109 - 116, XP002984610
TAKAHASHI H.: "PCR-SSCP-ho ni yoru histamine seiseikin no kenshutsu.dotei system no kaihatsu", NIPPON SHOKUHIN EISEI GAKKAI GAKUJUTSU KOENKAI KOEN YOSHISHU, vol. 48, 2002, pages 97, XP002984609
FUJII T.: "Heisei 7,8 nendo histamine seiseikin no jinsoku kenshutsu.dotei system no kaihatsu", SHOKUHIN ANZENSEI KOJO NI KANSURU KENKYU SEIKA HOKOKUSHU, 1999, pages 109 - 116, XP002984610
Attorney, Agent or Firm:
Ishikawa, Toru (24-12 Kanumadai 2-chom, Sagamihara-shi Kanagawa 33, JP)
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